您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

qHD5 encodes an AP2 factor that suppresses rice heading by down-regulating Ehd2 expression

文献类型： 外文期刊

作者： Sun, Bin 1 ; Xue, Pao 1 ; Wen, Xiao-Xia 1 ; Gong, Ke 1 ; Wang, Bei-Fang 1 ; Xu, Peng 1 ; Lin, Ze-Chuan 1 ; Peng, Ze-Qun 1 ; Fu, Jun-Lin 1 ; Yu, Ping 1 ; Sun, Lian-Ping 1 ; Zhang, Ying-Xin 1 ; Cao, Li-Ming 3 ; Cao, Li-Yong 1 ; Cheng, Shi-Hua 1 ; Wu, Wei-Xun 1 ; Zhan, Xiao-Deng 1 ;

作者机构： 1.China Natl Rice Res Inst, China Natl Ctr Rice Improvement, Hangzhou 310006, Peoples R China

2.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China

3.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201403, Peoples R China

4.Northern Ctr China Natl Rice Res Inst, Shuangyashan 155600, Peoples R China

5.Xuzhou Inst Agr Sci, Xuzhou 221131, Peoples R China

关键词： Rice; qHD5; AP2 transcription factor; Heading date; Adaptation

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 324 卷

页码：

收录情况： SCI

摘要： Heading date is crucial for rice reproduction and the geographical expansion of cultivation. We fine-mapped qHD5 and identified LOC_Os05g03040, a gene that encodes an AP2 transcription factor, as the candidate gene of qHD5 in our previous study. In this article, using two near-isogenic lines NIL(BG1) and NIL(XLJ), which were derived from the progeny of the cross between BigGrain1 (BG1) and Xiaolijing (XLJ), we verified that LOC_Os05g03040 represses heading date in rice through genetic complementation and CRISPR/Cas9 geneediting experiments. Complementary results showed that qHD5 is a semi-dominant gene and that the qHD5XLJ and qHD5BG1 alleles are both functional. The homozygous mutant line generated from knocking out qHD5XLJ in NIL(XLJ) headed earlier than NIL(XLJ) under both short-day and long-day conditions. In addition, the homozygous mutant line of qHD5BG1 in NIL(BG1) also headed slightly earlier than NIL(BG1). All of these results show that qHD5 represses the heading date in rice. Transient expression showed that the qHD5 protein localizes to the nucleus. Transactivation activity assays showed that the C-terminus is the critical site that affects self-activation in qHD5XLJ. qRT-PCR analysis revealed that qHD5 represses flowering by down-regulating Ehd2. qHD5 may have been selected during indica rice domestication.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台