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Genetic and molecular characterization of a novel reassortant H3N2 influenza virus from a sick pig in Eastern China in 2019

文献类型： 外文期刊

作者： Yang, Fan 1 ; Cheng, Linfang 1 ; Liu, Fumin 1 ; Yao, Hangping 1 ; Wu, Nanping 1 ; Xu, Lihua 3 ; Wu, Haibo 1 ;

作者机构： 1.Zhejiang Univ, Affiliated Hosp 1, Sch Med, State Key Lab Diag & Treatment Infect Dis, Hangzhou 310003, Peoples R China

2.Zhejiang Univ, Affiliated Hosp 1, Natl Clin Res Ctr Infect Dis, Sch Med, Hangzhou 310003, Peoples R China

3.Zhejiang Acad Agr Sci, Anim Husb & Vet Inst, Hangzhou 310021, Peoples R China

关键词： Swine influenza virus; reassortant; Eastern China

期刊名称：VETERINARY RESEARCH （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN： 0928-4249

年卷期： 2025 年 56 卷 1 期

页码：

收录情况： SCI

摘要： Swine influenza viruses (SIVs) cause clinical respiratory symptoms associated with high mortality rates among pigs. Because pigs can be a "mixing vessel" for influenza viruses, the SIV might pose a serious threat to animal and human health. In this study, an H3N2 SIV [A/swine/Zhejiang/19/2019(H3N2) (ZJ-SW19)] was isolated from a sick pig in Eastern China in 2019, and its molecular genetics were characterized. Phylogenetic analysis demonstrated the hemagglutinin (HA) and neuraminidase (NA) segments of ZJ-SW19 are highly homologous with those of H3N2 SIVs, belonging to human-like lineages; in contrast, the remaining six SIV segments (PB2, PB1, PA, NP, M, and NS) demonstrate the highest similarity with H1N1 SIVs isolated in East Asia during 2014-2020. The in vitro analysis of the virus's growth kinetics revealed that ZJ-SW19 can replicate efficiently in various mammalian and avian cell lines (including MDCK, A549, and DF-1). The receptor-binding analysis results indicated that ZJ-SW19 can bind to human-like receptors (alpha-2,6-linked sialic acid) and avian-like receptors (alpha-2,3-linked sialic acid). Moreover, ZJ-SW19 demonstrated significant differences compared with avian- and human-origin H3N2 influenza viruses in the antigenic analysis. Finally, in the pathogenicity test, ZJ-SW19 effectively replicated in the mouse lungs with moderate virulence. Therefore, continuous circulation of novel reassortant H3N2 SIVs indicates the need for long-term, close surveillance of influenza viruses in pig herds.

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