Quantum dot immunochromatographic strip for rapid and sensitive detection of H5 subtype avian influenza virus
文献类型： 外文期刊
作者： Wu, Han 1 ; Wang, Ping 1 ; Fu, Jiamin 1 ; Yang, Fan 1 ; Cheng, Linfang 1 ; Liu, Fumin 1 ; Yao, Hangping 1 ; Wu, Nanping 1 ; Xu, Lihua 3 ; Wu, Haibo 1 ; Li, Lanjuan 1 ;
作者机构： 1.Zhejiang Univ, Affiliated Hosp 1, Sch Med, State Key Lab Diag & Treatment Infect Dis, 79 Qingchun Rd, Hangzhou 310003, Zhejiang, Peoples R China
2.Zhejiang Univ, Affiliated Hosp 1, Natl Clin Res Ctr Infect Dis, Sch Med, 79 Qingchun Rd, Hangzhou 310003, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Anim Husb & Vet Inst, Hangzhou 310021, Peoples R China
关键词： Avian influenza virus; H5 subtype; Monoclonal antibodies; Quantum Dots; Fluorescent microsphere; Immunochromatographic strip
期刊名称：VIROLOGY JOURNAL （ 影响因子：3.8； 五年影响因子：3.7 ）
ISSN：
年卷期： 2025 年 22 卷 1 期
页码：
收录情况： SCI
摘要： The H5 subtype avian influenza viruses (AIVs) pose a major threat to wild fowl and poultry. Additionally, they can overcome the species barrier, inducing human infection, which may become fatal. Thus, the H5 subtype AIVs remain a global public health burden, with a huge pandemic potential. Therefore, it is imperative to establish a rapid, sensitive, and specific method for detecting H5 subtype AIV infection for diagnostic and preventive purposes. The quantum dot fluorescent microsphere-based immunochromatographic strip (QDFM-ICS) is being widely used for detecting various viruses. We here developed a pair of monoclonal antibodies (2F2 and 2B7) by immunizing mice with the A/duck/Zhejiang/6D2/2013(H5N6) virus and covalently linking them with quantum dots to generate a QDFM-ICS for detecting H5 subtype AIVs. The QDFM-ICS showed a limit of detection of 0.0625 hemagglutination units (HAU) per 80 mu L for live H5 subtype AIVs and 1.2 ng/mL purified H5N6 hemagglutination protein, respectively. In addition, it demonstrated good reproducibility with a coefficient of variation of < 10%, showing a high degree of repeatability. The strips exhibited a full percentage of specificity. Notably, their sensitivity and specificity remained unaffected even when stored for 3 months at room temperature or 4 degrees C. Furthermore, the application in practical testing on field samples demonstrated a strong correlation between QDFM-ICS and real-time PCR. The QDFM-ICS can be used in less time, with a simpler operation, and lesser expenditure. Thus, QDFM-ICS is a practical and promising technique for detecting H5 subtype AIVs, especially in point-of-care testing.
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