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Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR

文献类型： 外文期刊

作者： He, Chuan 1 ; Bai, Lan 1 ; Chen, Yifan 1 ; Jiang, Wei 1 ; Jia, Junwei 1 ; Pan, Aihu 2 ; Lv, Beibei 1 ; Wu, Xiao 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

3.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr, Shanghai 201106, Peoples R China

关键词： meat adulteration; food quality control; quantification; nucleic acid detection; droplet digital PCR

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

ISSN：

年卷期： 2022 年 11 卷 19 期

页码：

收录情况： SCI

摘要： In order to seek high profit, businesses mix beef and mutton with cheap meat, such as duck, pork, and chicken. Five pairs of primers were designed for quintuple droplet digital PCR (qddPCR) of specific genomic regions from five selected species and specificity and amplification efficiency were determined. The mixed DNA template with an equal copy number was used for detecting the accuracy and limit of multiplex PCR. The results showed that the primers and probes of the five selected species had good specificity with the minimum number of detection copies: 0.15 copies/mu L beef (Bos taurus), 0.28 copies/mu L duck (Anas platyrhynchos), 0.37 copies/mu L pork (Sus scrofa), 0.39 copies/mu L chicken (Gallus gallus), and 0.41 copies/mu L mutton (Ovis aries), respectively. The five sets of primers and probes could quickly judge whether the specified meat components existed in the food commodities.

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