DNA enrichment using deep eutectic solvents for improved PCR detection in Antarctic krill oil
文献类型： 外文期刊
作者： Su, Dong 1 ; Ma, Xinyu 1 ; Miao, Junkui 1 ; Liu, Xiaofang 1 ; Zhang, Yating 2 ; Zhao, Ling 2 ; Leng, Kailiang 1 ; Wang, Xixi 1 ; Yu, Yueqin 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China
2.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, State Key Lab Ecochem Engn, Qingdao 266042, Peoples R China
3.Univ New South Wales, Cluster Adv Macromol Design, Sch Chem Engn, Sydney, NSW 2052, Australia
4.Univ New South Wales, Australian Ctr Nanomed, Sch Chem Engn, Sydney, NSW 2052, Australia
5.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266200, Peoples R China
关键词： Euphausia superba; Krill oil; Deep eutectic solvents; 16S rRNA; Food authenticity
期刊名称：SEPARATION AND PURIFICATION TECHNOLOGY （ 影响因子：9.0； 五年影响因子：8.5 ）
ISSN： 1383-5866
年卷期： 2025 年 374 卷
页码：
收录情况： SCI
摘要： The commercialization of Antarctic krill oil raises concerns about product authenticity and adulteration. In this study, we developed a DNA-based authentication method for Antarctic krill oil. Species-specific PCR primers targeting a 157-bp fragment of the Euphausia superba 16S rRNA gene were designed, enabling high-specificity differentiation from Thunnus alalunga, Gadus ogac, and Salmo salar. The optimized PCR method achieved a detection sensitivity of 0.1 ng DNA/reaction. To address challenges in DNA extraction from complex lipid matrices, n-hexane and choline-based deep eutectic solvents (DES) were screened for their ability to separate interferents and enrich DNA. Among these, choline chloride-formic acid was selected as the optimal solvent due to its broader positive electrostatic potential (ESP) distribution (108.7 & Aring;(2), 56.0%). Post-PCR analysis successfully distinguished Antarctic krill oil from other edible oils. This work provides a robust tool for protecting high-value oils and advancing authenticity verification in related products.
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