文献类型： 外文期刊

作者： Garg, Vanika ¹ ; Dudchenko, Olga ² ; Wang, Jinpeng ⁴ ; Khan, Aamir W. ¹ ; Gupta, Saurabh ⁵ ; Kaur, Parwinder ⁶ ; Han, Kai ⁷ ; Saxena, Rachit K. ¹ ; Kale, Sandip M. ⁸ ; Pham, Melanie ² ; Yu, Jigao ⁴ ; Chitikineni, Annapurna ¹ ; Zhang, Zhikang ⁴ ; Fan, Guangyi ⁷ ; Lui, Christopher ² ; Valluri, Vinodkumar ¹ ; Meng, Fanbo ⁴ ; Bhandari, Aditi ¹ ; Liu, Xiaochuan ⁷ ; Yang, Tao ¹⁰ ; Chen, Hua ¹¹ ; Valliyodan, Babu ¹² ; Roorkiwal, Manish ¹ ; Shi, Chengcheng ⁷ ; Bin Yang, Hong ¹³ ; Durand, Neva C. ² ; Pandey, Manish K. ¹ ; Li, Guowei ¹⁵ ; Barmukh, Rutwik ¹ ; Wang, Xingjun ¹⁵ ; Chen, Xiaoping ¹³ ; Lam, Hon-Ming ¹⁶ ; Jiang, Huifang ¹⁸ ; Zong, Xuxiao ¹⁰ ; Liang, Xuanqiang ¹³ ; Liu, Xin ⁷ ; Liao, Boshou ¹⁸ ; Guo, Baozhu ²⁰ ; Jackson, Scott ²¹ ; Nguyen, Henry T. ²² ; Zhuang, Weijian ¹¹ ; Shubo, Wan ¹⁵ ; Wang, Xiyin ⁴ ; Aiden, Erez Lieberman ² ; Bennetzen, Jeffrey L. ²⁶ ; Varshney, Rajeev K. ¹ ;

作者机构： 1.Int Crops Res Inst Semi Arid Trop, Ctr Excellence Genom & Syst Biol, Hyderabad, India

2.Baylor Coll Med, Dept Mol & Human Genet, Ctr Genome Architecture, Houston, TX 77030 USA

3.Rice Univ, Ctr Theoret Biol Phys, Houston, TX USA

4.North China Univ Sci & Technol, Sch Life Sci, Tangshan, Peoples R China

5.Max Planck Inst Mol Plant Physiol, Potsdam, Germany

6.Univ Western Australia, UWA Sch Agr & Environm, Perth, WA, Australia

7.BGI Shenzhen, BGI Qingdao, Qingdao 266555, Peoples R China

8.Leibniz Inst Plant Genet & Crop Plant Res IPK, Gatersleben, Germany

9.BGI Shenzhen, Shenzhen 518083, Peoples R China

10.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Inst Crop Sci, Beijing 100081, Peoples R China

11.Fujian Agr & Forestry Univ, Ctr Legume Oil Crop Genet & Syst Biol, Inst Oil Crops, Fuzhou, Peoples R China

12.Lincoln Univ, Dept Agr & Environm Sci, Jefferson City, MO USA

13.Guangdong Acad Agr Sci, Crops Res Inst, Guangzhou, Peoples R China

14.Shandong Acad Agr Sci, Shandong Peanut Res Inst, Qingdao, Peoples R China

15.Shandong Acad Agr Sci, Biotechnol Res Ctr, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan, Peoples R China

16.Chinese Univ Hong Kong, Ctr Soybean Res, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China

17.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Peoples R China

18.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Oil Crops Res Inst, Wuhan, Peoples R China

19.BGI Shenzhen, China Natl GeneBank, Shenzhen 518120, Peoples R China

20.USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA

21.Bayer Crop Sci, Chesterfield, MO USA

22.Univ Missouri, Div Plant Sci, Columbia, MO USA

23.Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO USA

24.ShanghaiTech, Shanghai Inst Adv Immunochem Studies, Pudong, Peoples R China

25.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

26.Univ Georgia, Dept Genet, Athens, GA 30602 USA

27.Murdoch Univ, State Agr Biotechnol Ctr, Ctr Crop & Food Innovat, Food Futures Inst, Murdoch, WA, Australia

28.Univ Western Australia, UWA Inst Agr, Perth, WA, Australia

关键词： Evolution; Plant genomes; GWAS; Nodulation; QTL; Legumes

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：10.7； 五年影响因子：11.4 ）

ISSN： 2090-1232

年卷期： 2022 年 42 卷

页码：

收录情况： SCI

摘要： Introduction: Legume crops are an important source of protein and oil for human health and in fixing atmospheric N2 for soil enrichment. With an objective to accelerate much-needed genetic analyses and breeding applications, draft genome assemblies were generated in several legume crops; many of them are not high quality because they are mainly based on short reads. However, the superior quality of gen-ome assembly is crucial for a detailed understanding of genomic architecture, genome evolution, and crop improvement. Objectives: Present study was undertaken with an objective of developing improved chromosome-length genome assemblies in six different legumes followed by their systematic investigation to unravel differ-ent aspects of genome organization and legume evolution.Methods: We employed in situ Hi-C data to improve the existing draft genomes and performed different evolutionary and comparative analyses using improved genome assemblies.Results: We have developed chromosome-length genome assemblies in chickpea, pigeonpea, soybean, subterranean clover, and two wild progenitor species of cultivated groundnut (A. duranensis and A. ipaen-sis). A comprehensive comparative analysis of these genome assemblies offered improved insights into various evolutionary events that shaped the present-day legume species. We highlighted the expansion of gene families contributing to unique traits such as nodulation in legumes, gravitropism in groundnut, and oil biosynthesis in oilseed legume crops such as groundnut and soybean. As examples, we have demonstrated the utility of improved genome assemblies for enhancing the resolution of "QTL-hotspot" identification for drought tolerance in chickpea and marker-trait associations for agronomic traits in pigeonpea through genome-wide association study. Genomic resources developed in this study are pub-licly available through an online repository, 'Legumepedia'.Conclusion: This study reports chromosome-length genome assemblies of six legume species and demon-strates the utility of these assemblies in crop improvement. The genomic resources developed here will have significant role in accelerating genetic improvement applications of legume crops.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).