A Single-Nucleus Resolution Atlas of Transcriptome and Chromatin Accessibility for Peanut (Arachis Hypogaea L.) Leaves
文献类型： 外文期刊
作者： Liu, Hao 1 ; Guo, Zenhua 2 ; Gangurde, Sunil S. 3 ; Garg, Vanika 4 ; Deng, Quanqing 1 ; Du, Puxuan 1 ; Lu, Qing 1 ; Chitikineni, Annapurna 4 ; Xiao, Yuan 1 ; Wang, Wenyi 5 ; Hong, Yanbin 1 ; Varshney, Rajeev K. 4 ; Chen, Xiaoping 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Crops Res Inst, Guangdong Prov Key Lab Crop Genet Improvement, South China Peanut Sub Ctr,Natl Ctr Oilseed Crops, Guangzhou 510640, Guangdong, Peoples R China
2.Heilongjiang Acad Agr Sci, Rice Res Inst, Jiamusi 154026, Heilongjiang, Peoples R China
3.Univ Georgia, Dept Plant Pathol, Crop Genet & Breeding Res Unit, USDA ARS, Tifton, GA 31793 USA
4.Murdoch Univ MU, Food Futures Inst, State Agr Biotechnol Ctr, Ctr Crop & Food Innovat, Murdoch, WA 6150, Australia
5.South China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China
关键词： chromatin accessibility; groundnut; leaf development; plant single-cell multi-omics; single nuclei
期刊名称：ADVANCED BIOLOGY （ 影响因子：3.7； 五年影响因子：3.7 ）
ISSN： 2701-0198
年卷期： 2023 年
页码：
收录情况： SCI
摘要： The peanut is an important worldwide cash-crop for edible oil and protein. However, the kinetic mechanisms that determine gene expression and chromatin accessibility during leaf development in peanut represented allotetraploid leguminous crops are poorly understood at single-cell resolution. Here, a single-nucleus atlas of peanut leaves is developed by simultaneously profiling the transcriptome and chromatin accessibility in the same individual-cell using fluorescence-activated sorted single-nuclei. In total, 5930 cells with 50 890 expressed genes are classified into 18 cell-clusters, and 5315 chromatin fragments are enriched with 26 083 target genes in the chromatin accessible landscape. The developmental trajectory analysis reveals the involvement of the ethylene-AP2 module in leaf cell differentiation, and cell-cycle analysis demonstrated that genome replication featured in distinct cell-types with circadian rhythms transcription factors (TFs). Furthermore, dual-omics illustrates that the fatty acid pathway modulates epidermal-guard cells differentiation and providescritical TFs interaction networks for understanding mesophyll development, and the cytokinin module (LHY/LOG) that regulates vascular growth. Additionally, an AT-hook protein AhAHL11 is identified that promotes leaf area expansion by modulating the auxin content increase. In summary, the simultaneous profiling of transcription and chromatin accessibility landscapes using snRNA/ATAC-seq provides novel biological insights into the dynamic processes of peanut leaf cell development at the cellular level.
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