文献类型： 外文期刊

作者： Chen, Junkui ¹ ; Li, Zhengpeng ² ; Wang, Xiaofan ³ ; Fan, Bin ⁴ ; Deng, Feilong ³ ; Hongwei, D. Yu ⁵ ; Ze, Xiaolei ⁶ ; Zhu, Liying ¹ ; Yin, Yeshi ⁷ ; Chen, Yanhong ⁸ ; Zhao, Jiangchao ³ ; Yang, Yunsheng ² ; Wang, Xin ¹ ;

作者机构： 1.Inst Food Sci, Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

2.Chinese Peoples Liberat Army Gen Hosp, Dept Gastroenterol & Hepatol, Beijing, Peoples R China

3.Univ Arkansas, Dept Anim Sci, Div Agr, Fayetteville, AR 72701 USA

4.Hangzhou Med Coll, Zhejiang Prov Peoples Hosp, Dept Crit Care Med, Peoples Hosp, Hangzhou, Peoples R China

5.Marshall Univ, Dept Biomed Sci, Huntington, WV USA

6.BYHEALTH CO Ltd, Guangzhou, Peoples R China

7.Hunan Univ Sci & Engn, Yongzhou, Hunan, Peoples R China

8.Zhejiang Univ, Lab Anim Ctr, Hangzhou, Peoples R China

关键词： Prevotella; isomalto-oligosaccharides; microbiome; short chain fatty acids

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：9.043； 五年影响因子：8.113 ）

ISSN： 2165-0497

年卷期：

页码：

收录情况： SCI

摘要： The Prevotella enterotype type is a human traditional enterotype with high dietary fiber intake, which is related to healthy ageing and Parkinson's disease development. Manipulations of the dwelled gut microbes by dietary isomalto-oligosaccharides efficiently sustained Prevotella type enterotypes, indicating that it can be used in the improvement of elderly health by increasing the gut transit time. The human digestive tract is colonized by trillions of bacterial cells that play important roles in human health and diseases. It is well known that dietary habits are associated with human microbiota enterotypes. However, the factors that determine the enterotype still remain elusive. In this study, it was first examined, via in vitro batch fermentation, how different carbohydrates affect the Bacteroides and Prevotella enterotypes. Among the 11 substrates (fructo-, galacto-, xylo-, manno-, and isomalto-oligosaccharides [IMO] and lactulose, raffinose, starch, inulin [INU], mannitol, and xylitol) tested, IMO, INU, and starch were found to sustain the growth of Prevotella through batch fermentation. The development of the Prevotella and Bacteroides enterotypes was further simulated in chemostats using fecal samples. IMO coupled with faster dilution rates and lower pH were required to sustain the growth of Prevotella copri in the chemostat based on 16S rRNA gene and metagenomic sequencing. Meanwhile, starch with relatively lower dilution rates and higher pH was required to support the development of the Bacteroides enterotype. Amylo-alpha-1,6-glucosidase, pectin, and xylan lyases were the carbohydrate-active enzymes associated with the Prevotella enterotype. The Bacteroides enterotype was associated with more diversified carbohydrate-active enzymes. Consistently, since honey contains high isomaltose content, mice fed IMO and honey displayed an increased relative abundance of Prevotella in the colon. In conclusion, both in vitro systems and a mouse model were used to demonstrate that IMO maintains the Prevotella enterotype. This result provides insight into the nutritional requirements underlying gut enterotype formation.IMPORTANCE The Prevotella enterotype type is a human traditional enterotype with high dietary fiber intake, which is related to healthy ageing and Parkinson's disease development. Manipulations of the dwelled gut microbes by dietary isomalto-oligosaccharides efficiently sustained Prevotella type enterotypes, indicating that it can be used in the improvement of elderly health by increasing the gut transit time.