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Silencing of Cytochrome P450 genes CYP6CY14 and CYP6DC1 in Aphis gossypii by RNA interference enhances susceptibility to clothianidin

文献类型： 外文期刊

作者： Ullah, Farman 1 ; Gul, Hina 2 ; Tariq, Kaleem 3 ; Hafeez, Muhammad 1 ; Desneux, Nicolas 4 ; Song, Dunlun 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

2.China Agr Univ, Coll Plant Protect, Dept Entomol, Beijing 100193, Peoples R China

3.Abdul Wali Khan Univ Mardan, Dept Entomol, Khyber Pakhtunkhwa, Pakistan

4.Univ Cote Azur, INRAE, CNRS, UMR ISA, F-06000 Nice, France

关键词： Cotton aphid; Neonicotinoid; Cytochrome P450 monooxygenase; Insecticide resistance; Resistance mechanism

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：6.9； 五年影响因子：5.2 ）

ISSN： 0171-8177

年卷期： 2023 年 43 卷 3 期

页码：

收录情况： SCI

摘要： Cytochrome P450s (CYPs) genes are associated with insecticide resistance through overexpression and detoxification mechanisms in several insect-pest species. However, little is known about the role of P450s genes and their response to clothianidin resistance development in cotton-melon aphid, Aphis gossypii, a serious crop pest. In this study, we characterized CYP6CY14 and CYP6DC1 from CYP3 clad that are differentially expressed in aphids. Quantitative real-time PCR (qRT-PCR) analyses revealed that CYP6CY14 and CYP6DC1 expressions were significantly higher among thirty five cytochrome P450 genes in clothianidin-resistant strain (CT-R) of A. gossypii compared to the susceptible strain (CT-S). RNAi was conducted to knock-down the highly expressed CYPs genes (CYP6CY14 and CYP6DC1 from CYP3 clad) and study their detoxification effects on clothianidin. The apterous adult aphids became more sensitive to clothianidin, with a higher mortality rate than the control after silencing CYP6CY14 and CYP6DC1 genes. Bioinformatic analysis showed conserved motifs in amino acid sequences of CYP6CY14 and CYP6DC1 with other insect species. This study highlighted the functional importance of these P450 genes in insecticide resistance development and revealed that overexpression of CYP6CY14 and CYP6DC1 genes was responsible for the high levels of insecticide resistance in the A. gossypii population.

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