文献类型： 外文期刊

作者： Zhao, Shaoguan ¹ ; Liu, Chen ² ; Yuan, Ying ² ; Zhao, Qingyun ³ ; Zhang, Zhiyang ¹ ; Ren, Xiangyu ¹ ; Yue, Yang ² ; Sun, Shuo ² ; Sun, Shiqi ² ; Zhang, Qi ² ; Xing, Guangnan ⁴ ; Wang, Ming ⁶ ; Xiong, Wu ¹ ; Shen, Qirong ² ;

作者机构： 1.Nanjing Agr Univ, Sanya Inst, Sanya 572000, Hainan, Peoples R China

2.Nanjing Agr Univ, Jiangsu Prov Key Lab Solid Organ Waste Utilizat, Collaborat Innovat Ctr Solid Organ Wastes,Key Lab, Educ Minist Engn Ctr Resource-Saving Fertilizers, Nanjing 210095, Peoples R China

3.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Hainan 571500, Wanning, Peoples R China

4.Natl Key Lab Crop Genet Nanjing Agr Univ, Soybean Res Inst,Natl Key Lab Crop Genet, MARA Natl Ctr Soybean Improvement, MARA Key Lab Biol & Genet Improvement Soybean, Nanjing 210095, Peoples R China

5.Nanjing Agr Univ, Coll Agr, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Germplasm Enhancement & Utilizat, Nanjing 210095, Peoples R China

6.Nanjing Agr Univ, Key Lab Soybean Dis & Pest Control, Minist Agr & Rural Affairs, Nanjing 210095, Peoples R China

关键词： Crop domestication; Rhizosphere microbiome; Protist and Bacteria; Generalists and specialists

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.9； 五年影响因子：7.2 ）

ISSN： 0944-5013

年卷期： 2025 年 301 卷

页码：

收录情况： SCI

摘要： Crop domestication has long been known to reshape rhizosphere microbial communities, yet research has focused disproprotionately on bacteria and fungal responses to crop domestication while neglecting protist communities. Protists, as key microbial predators regulating bacterial populations and thereby their functionalities, remain understudied in this context. Here, we investigate the influence of soybean domestication on both bacterial and protist communities, with a focus on the reorganization of ecological strategies, specifically generalists and specialists, within these microbiomes. We analyzed 270 rhizosphere samples from 27 domesticated and 63 wild soybean varieties. Domestication significantly altered community compositions of bacterial communities, with wild soybeans harboring higher proprotions of Pseudomonadota (71.4 %) and Bacillota (4.8 %), while domesticated soybeans exhibited an enrichment of Bacteroidota (11.0 %). Protist communities also diverged: wild soybeans were dominated by Cercozoa (58.2 %) and Gyrista (23.5 %), while domesticated plants had more Ciliophora (7.1 %) and Evosea (5.7 %). Domesticated soybeans hosted fewer generalist and specialist bacteria but more generalist protists, suggesting divergent microbial responses to domestication. Correlation analyses revealed that bacterial and protist generalists exhibited strong positive correlations with each other. At the same time, bacterial and protist specialists also showed positive correlations in wild soybeans-patterns that were largely absent in their domesticated counterparts. Functionally, wild soybeans supported more ureolytic and methylotrophic bacteria, while domesticated soybeans favored nitrate-respiration taxa. Notably, predatory protists in wild soybeans were significantly correlated with bacteria involved in carbon and nitrogen cycling, a key ecological relationship lost with domestication. These findings suggest that domestication exerts different selection pressures on bacteria and protists, disrupting potential relationships between bacterial and protist functional groups.