文献类型： 外文期刊

作者： He, Chengyong ¹ ; Xing, Fei ³ ; Liang, Jiahui ⁴ ; Zhang, Zhixiang ² ; Zhan, Binhui ² ; Habili, Nuredin ⁵ ; Wang, Hongqing ¹ ; Li, Shifang ² ;

作者机构： 1.China Agr Univ, Coll Hort, Dept Fruit Sci, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Southwest Univ, Citrus Res Inst, Natl Citrus Engn Res Ctr, Chongqing 400712, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Inst Grassland Flowers & Ecol, Beijing 100097, Peoples R China

5.Univ Adelaide, Australian Wine Res Inst Waite Precinct, Adelaide 5000, Australia

关键词： ABA; ABI5; apple necrotic mosaic virus; ATPO; mitochondrial ATP synthase; OSCP

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年 74 卷 14 期

页码：

收录情况： SCI

摘要： Apple necrotic mosaic virus (ApNMV) is associated with apple mosaic disease in China. However, the mechanisms of ApNMV infection, as well as host defence against the virus, are still poorly understood. Mitochondrial ATP synthase plays a fundamental role in the regulation of plant growth and development. However, mitochondrial ATP synthase function in response to virus infection remains to be defined. In the present study, a yeast two-hybrid (Y2H) screening revealed that the apple mitochondrial ATP synthase oligomycin sensitivity-conferring protein (OSCP) subunit (MdATPO) interacts with ApNMV coat protein (CP). It was further verified that overexpression of MdATPO in Nicotiana benthamiana inhibited viral accumulation. In contrast, silencing of NbATPO facilitated viral accumulation, indicating that ATPO plays a defensive role during ApNMV infection. Further investigation demonstrated that ApNMV infection accelerated abscisic acid (ABA) accumulation, and ABA negatively regulated ATPO transcription, which was related to the ability of ABA insensitive 5 (ABI5) to bind to the ABA-responsive elements (ABREs) of the ATPO promoter. Taken together, our results indicated that transcription factor ABI5 negatively regulated ATPO transcription by directly binding to its promoter, leading to the susceptibility of apple and N. benthamiana to ApNMV infection. The current study facilitates a comprehensive understanding of the intricate responses of the host to ApNMV infection. ApNMV infection stimulates ABA accumulation in the host, which negatively regulates mitochondrial ATPOtranscription by ABI5 binding to its promoter.