Identification of suitable reference genes for quantitative reverse transcription PCR in Luffa (Luffa cylindrica)
文献类型： 外文期刊
作者： Zhao, Gangjun 1 ; Wang, Meng 3 ; Gan, Yaqin 3 ; Gong, Hao 1 ; Li, Junxing 1 ; Zheng, Xiaoming 1 ; Liu, Xiaoxi 1 ; Zhao, Siying 3 ; Luo, Jianning 1 ; Wu, Haibin 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
3.Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Peoples R China
关键词： Luffa; Sponge gourd; RT-qPCR; Reference genes; PP2A; Sex determination
期刊名称：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS （ 影响因子：3.023； 五年影响因子：3.442 ）
ISSN： 0971-5894
年卷期： 2022 年 28 卷 4 期
页码：
收录情况： SCI
摘要： Reverse transcription real-time quantitative PCR is widely used to quantify gene expression. Reference genes are usually used as internal controls to measure the target gene expression level. To date, there is no consensus on the use of systematically validated reference genes in different tissues of Luffa. This study evaluated the expression stability of 11 candidate reference genes in different tissues using five algorithms (BestKeeper, comparative delta-Ct method, GeNorm, NormFinder, and RefFinder). Protein phosphatase 2A was the most stable gene, while alpha Tubulin was the least stable. The relative expression of ethylene-related genes in different tissues was also analyzed to reveal their role in sex determination. This study provides the basis for using suitable reference genes to evaluate targeted gene expression.
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