Sigma factor 70 RpoD contributes to virulence by regulating cell motility, oxidative stress tolerance, and manipulating the expression of hrpG and hrpX in Xanthomonas oryzae pv. oryzae
文献类型： 外文期刊
作者： Xu, Zhizhou 1 ; Wu, Guichun 4 ; Wang, Bo 3 ; Guo, Baodian 3 ; Sheng, Cong 3 ; Zhao, Yangyang 3 ; Tang, Bao 3 ; Zhao, Yancun 3 ; Liu, Fengquan 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Peoples R China
2.Guizhou Univ, Coll Agr, Dept Plant Pathol, Key Lab Agr Microbiol, Guiyang 550025, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base Minist Sci & Technol, Nanjing 210014, Peoples R China
4.Anhui Agr Univ, Sch Life Sci, Natl Engn Lab Crop Stress Resistance Breeding, Hefei 230036, Peoples R China
关键词： Oryza sativa; sigma factor; pathogenicity; transcriptional regulation; type III secretion system
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.4； 五年影响因子：4.8 ）
ISSN： 2095-3119
年卷期： 2025 年 24 卷 5 期
页码：
收录情况： SCI
摘要： Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight in rice, which reduces crop yield and leads to significant economic losses. Bacterial sigma (sigma) factors are highly specialized proteins that allow RNA polymerase to recognize and bind to specific promoters. sigma 70 factors also regulate the expression of genes involved in stress response and virulence. However, the role of RpoD in Xoo is still unclear. In this study, we found that sigma 70 factor RpoD is quite conservative among phytopathogenic bacteria, especially in Xanthomonas sp. In Xoo, PXO_RpoD plays an important role in oxidative stress tolerance and cell motility, as well as being essential for full virulence. Cleavage under targets and tagmentation (CUT&Tag) analyses indicated that RpoD mediates the type three secretion system (T3SS) by regulating the regulation of hrpG and hrpX. By performing bacterial one-hybrid and electrophoretic mobility assay (EMSA), we observed that RpoD directly bound to the promoters of hrpG and hrpX. Collectively, these results demonstrate the transcriptional mechanism and pathogenic functions of RpoD in regulating cell motility and oxidative stress response, providing novel insights into potential targets for
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