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Mechanism of Eriochloa villosa (Thunb.) Kunth Resistance to Nicosulfuron

文献类型： 外文期刊

作者： Guo, Jing 1 ; Xu, Zeqian 2 ; Jiao, Ting 1 ; Gao, Hong 1 ; Wang, Yuechao 4 ; Zhang, Liguo 4 ; Li, Mukai 5 ; Liu, Xiaomin 6 ; Yan, Chunxiu 1 ; Han, Yujun 1 ;

作者机构： 1.Northeast Agr Univ, Coll Plant Protect, Harbin 150030, Peoples R China

2.Shanghai Jiao Tong Univ, State Key Lab Oncogenes, Shanghai 200240, Peoples R China

3.Shanghai Jiao Tong Univ, Bioid Ctr, Sch Biomed Engn, Shanghai 200240, Peoples R China

4.Heilongjiang Acad Agr Sci, Crop Resources Inst, Harbin 150086, Peoples R China

5.Heilongjiang Bayi Agr Univ, Coll Agr, Daqing 163319, Peoples R China

6.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Key Lab Crop Cultivat Physiol & Green Prod Hebei P, Shijiazhuang 050035, Peoples R China

关键词： nicosulfuron; E. villosa; metabolic resistance; transcriptome

期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）

ISSN：

年卷期： 2024 年 14 卷 10 期

页码：

收录情况： SCI

摘要： Eriochloa villosa (Thunb.) Kunth, the main weed in corn fields, has gradually developed resistance to nicosulfuron due to continuous and extensive application. We identified a biotype showing resistance to ALS inhibitor nicosulfuron with a resistant index 13.83, but without any target spot mutation. Herein, transcriptome sequencing was used to analyze the differences in gene expression at the transcriptional level between nicosulfuron-resistant E. villosa HEK-40 varieties and sensitive E. villosa HEK-15 varieties. The resistant and sensitive varieties comparison revealed 9931 DEGs after nicosulfuron application, of which 5426 and 4505 genes were up-regulated and down-regulated, respectively. Some contigs related to metabolic resistance were identified based on differential expression via RNA-Seq, which includes ABC transporters (ko02010), glucosinolate biosynthesis (ko00966), 2-oxocarboxylic acid metabolism (ko01210), alanine, aspartate, and glutamate metabolism pathways (ko00250). Seven CYP450 genes, four GST genes, ten ABC transporter genes, and two GT genes related to metabolic resistance were identified. The 10 candidate genes screened were validated using q-PCR. This validation indicates that activities associated with P450 enzymes, ABC transporters, and glutathione S-transferases (GST) may play a role in conferring resistance, which is important for reducing the impact of weeds on corn fields and ensuring food security.

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