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Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment

文献类型： 外文期刊

作者： Chen, He 1 ; Liu, Hao 3 ; Ji, Yanran 4 ; Sha, Zekun 4 ; An, Li 1 ; Li, Meng 1 ; Zhang, Di 1 ; Wu, Xujin 1 ; Hua, Xiude 4 ;

作者机构： 1.Henan Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Zhengzhou 450002, Peoples R China

2.Key Lab Grain Qual & Safety & Testing Henan Prov, Zhengzhou 450002, Peoples R China

3.Henan Univ, Sch Life Sci, Kaifeng 475004, Peoples R China

4.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

关键词： pyridaben; monoclonal antibody; colorimetric lateral flow immunoassay; enzyme-linked immunosorbent assay; on-site detection

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN：

年卷期： 2023 年 13 卷 5 期

页码：

收录情况： SCI

摘要： Pyridaben, a broad-spectrum pyridazinone acaricide that is widely used in agricultural production, can induce neurotoxicity and reproductive abnormalities, and is highly toxic to aquatic organisms. In this study, a pyridaben hapten was synthesized and used to prepare monoclonal antibodies (mAbs), among which 6E3G8D7 showed the highest sensitivity in indirect competitive enzyme-linked immunosorbent assay, with a 50% inhibitory concentration (IC50) of 3.49 ng mL(-1). The mAb, 6E3G8D7, was further applied to a gold nanoparticle-based colorimetric lateral flow immunoassay (CLFIA) for pyridaben detection, according to the signal intensity ratio of the test line to the control line, which showed a visual limit of detection of 5 ng mL(-1). The CLFIA also showed high specificity and achieved excellent accuracy in different matrices. In addition, the amounts of pyridaben in blind samples detected by the CLFIA, were consistent with high-performance liquid chromatography. Therefore, the developed CLFIA is considered a promising, reliable, and portable method for pyridaben on-site detection in agro-products and environmental samples.

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