您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Exposure to benzo(a)pyrene interfered with cell composition and immune ability of common carp (Cyprinus carpio) intestine through inducing cell heterogeneous responses

文献类型： 外文期刊

作者： Hao, Qirui 1 ; Wang, Peng 1 ; Qin, Dongli 1 ; Chen, Zhongxiang 1 ; Li, Chenhui 1 ; Huang, Li 1 ; Wu, Song 1 ; Yang, Jixian 5 ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Control Qual & Safety Aquat Prod, Beijing 100141, Peoples R China

3.Minist Agr & Rural Affairs, Supervis Inspect & Testing Ctr Fishery Environm &, Harbin 150070, Peoples R China

4.Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Aquat Prod Harbi, Harbin 150070, Peoples R China

5.Harbin Inst Technol, Harbin 150006, Peoples R China

关键词： Benzo( a )pyrene exposure; Common carp; Epithelial cells heterogeneity; T_cells heterogeneity; Lysosome-phagosome pathway

期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2024 年 579 卷

页码：

收录情况： SCI

摘要： The intricate heterogeneity of fish intestinal cell types response to environmental stressors has remained largely unaddressed due to previous technological limitations. Emerging single-cell sequencing technology (scRNA-seq), derived from the 2010s, may be a key breakthrough in this area, which could accurately obtain the transcriptome of each cell. In this context, common carp (Cyprinus carpio), which takes the intestine as the main immune organ, was selected as the test subject in this study. The study, mainly based on single-cell sequencing technology and supplemented by gas chromatography mass spectrometry (GC/MS), histological sectioning techniques and transcriptome analysis, revealed the detailed heterogeneity responses of intestinal cells in common carp after benzo(a)pyrene (B[a]P) exposure. Our findings showed a dose-dependent effect on B[a]P accumulation within the common carp intestine as the B[a]P concentration increased. B[a]P exposure led to mucous lamina propria edema and confluent intestinal villi. The high concentration group exhibited uneven and adherent intestinal villi, an abnormal decrease in mucosal epithelial cells, vacuolation, and an anomalously widened base thickness.In the intestinal atlas, an increased proportion of T_cells and a decreased proportion of ECs were identified as the most significant features. These changes primarily affected the intestine by impairing development processes and boosting immunocompetence. A comprehensive analysis of the heterogeneous responses of ECs and T_cells to B [a]P exposure revealed that the lysosome-phagosome pathway, strengthened by the "Lysosome" pathway in the EC1 subset and the "Phagosome" pathway in T_cell 2 subset, might be one of the most critical defense mechanisms within the common carp's intestine. In conclusion, our research presents the first description of the intestinal atlas and the heterogeneous responses in common carp following B[a]P exposure. Our findings enrich the understanding of the impact of polyaromatic hydrocarbons, exemplified by B[a]P, on cyprinidae fish.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台