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Fine mapping and analysis of candidate genes for qBT2andqBT7.2 locus controlling bolting time in radish (Raphanussativus L.)

文献类型： 外文期刊

作者： Jin, Yueyue 1 ; Luo, Xiaobo 2 ; Li, Yadong 1 ; Peng, Xiao 1 ; Wu, Linjun 1 ; Yang, Guangqian 1 ; Xu, Xiuhong 1 ; Pei, Yun 1 ; Li, Wei 1 ; Zhang, Wanping 1 ;

作者机构： 1.Guizhou Univ, Coll Agr, Guiyang 550003, Guizhou, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Inst Biotechnol, Guiyang 550006, Guizhou, Peoples R China

3.Guizhou Univ, Inst Vegetable Ind Technol Res, Guiyang 550003, Peoples R China

4.Guizhou Univ, Guizhou Higher Educ Facil Vegetable Engn Reseach C, Guiyang 550003, Guizhou, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 1 期

页码：

收录情况： SCI

摘要： Key messageTwo major QTLs for bolting time in radish were mapped to chromosome 02 and 07 in a 0.37 Mb and 0. 52 Mb interval, RsFLC1 and RsFLC2 is the critical genes.AbstractRadish (Raphanussativus L.) is an important vegetable crop of Cruciferae. The premature bolting and flowering reduces the yield and quality of the fleshy root of radish. However, the molecular mechanism underlying bolting and flowering in radish remains unknown. In YZH (early bolting) x XHT (late bolting) F2 population, a high-density genetic linkage map was constructed with genetic distance of 2497.74 cM and an average interval of 2.31 cM. A total of nine QTLs for bolting time and two QTLs for flowering time were detected. Three QTLs associated with bolting time in radish were identified by QTL-seq using radish GDE (early bolting) x GDL (late bolting) F-2 population. Fine mapping narrowed down qBT2 and qBT7.2 to an 0.37 Mb and 0.52 Mb region on chromosome 02 and 07, respectively. RNA-seq and qRT-PCR analysis showed that RsFLC1 and RsFLC2 were the candidate gene for qBT7.2 and qBT2 locus, respectively. Subcellular localization exhibited that RsFLC1 and RsFLC2 were mainly expressed in the nucleus. A 1856-bp insertion in the first intron of RsFLC1 was responsible for bolting time. Overexpression of RsFLC2 in Arabidopsis was significantly delayed flowering. These findings will provide new insights into the exploring the molecular mechanism of late bolting and promote the marker-assisted selection for breeding late-bolting varieties in radish.

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