Cloning, tissue specificity and regulation of expression of genes of four key enzymes related to long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis by ambient salinity during embryogenesis in the marine teleost Siganus guttatus
文献类型： 外文期刊
作者： You, Cuihong 1 ; Miao, Shuangshuang 1 ; Xie, Zhiyong 2 ; Lin, Siyuan 2 ; Wang, Shuqi 2 ; Chen, Cuiying 2 ; Lin, Li 1 ; Huang, Yanhua 1 ; Zhou, Meng 1 ; Dong, Yewei 1 ; Li, Yuanyou 3 ; Zhuang, Ping 5 ;
作者机构： 1.Zhongkai Univ Agr & Engn, Innovat Inst Anim Hlth Breeding, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Guangdong, Peoples R China
2.Shantou Univ, Guangdong Prov Key Lab Marine Biotechnol, Shantou 515063, Guangdong, Peoples R China
3.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Peoples R China
4.South China Agr Univ, Univ Joint Lab Guangdong Prov Hong Kong & Macao Re, Guangzhou 510642, Peoples R China
5.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
关键词： Fatty acyl desaturase; Elongation of very long-chain fatty acid; Fertilized eggs; Salinity treatment
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）
ISSN： 1096-4959
年卷期： 2024 年 269 卷
页码：
收录情况： SCI
摘要： The rabbitfish Siganus canaliculatus was the first marine teleost reported to possess long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic ability, and the related molecular mechanisms have been well clarified. Here, we investigated the LC-PUFA biosynthesis capability of the congeneric rabbitfish S. guttatus. First, cDNAs of genes for four key enzymes related to LC-PUFA biosynthesis, namely A6A5 fatty acyl desaturase (fads2) (1335 bp; 445 aa), A4 fads2 (1335 bp; 445 aa), and elongation of very long chain fatty acid proteins (elovl5) (873 bp; 291 aa) and elovl4 (906 bp; 302 aa) were cloned from the liver of S. guttatus. The A6A5 fads2, A4 fads2 and elovl5 genes showed high expression in brain, liver, spleen, gallbladder, and intestine but relatively low expression in eye, whereas the elovl4 gene showed specific and high expression in eye. During embryogenesis, mRNA expression of A4 fads2 and elovl4 was detected from 8 h post-fertilization (hpf) and then maintained a high level to 24 hpf, while mRNA expression of A6A5 fads2 and elovl5 reached a peak at 14 hpf but then declined. In addition, ambient salinity (32 ppt and 20 ppt) exerted some regulatory influence on the expression of the four genes during embryogenesis. The levels of C18 PUFA precursors and, especially, PUFA and DHA of the embryos, decreased from 17 hpf to 24 hpf. These results suggested that S. guttatus, similar to the congeneric S. canaliculatus, would have capability for LC-PUFA biosynthesis, which is still not activated at the fertilized egg stage.
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