您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion

文献类型： 外文期刊

作者： Liu, Hao 1 ; Liu, Yan-zhen 1 ; Zheng, Shao-quan 2 ; Jiang, Ji-mou 2 ; Wang, Ping 3 ; Chen, Wei 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

2.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Hort Sci, Fuzhou 350002, Peoples R China

4.Fujian Agr & Forestry Univ, Key Lab, Minist Educ Genet, Fuzhou 350002, Peoples R China

关键词： Longan;Seed abortion;Proteomics;2-DE;MALDI-TOF-MS/MS

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Two-dimensional gel electrophoresis (2-DE), coupled with mass spectroscopy, was used to study seed abortion in Dimocarpus longan Lour. (cv. Minjiao 64-1) by comparing normal and aborted seeds at three developmental stages. More than 1,000 protein spots were reproducibly detected in 2-DE gels, with 43 protein spots being significantly altered in their intensity between normal and aborted seeds at least at one stage. Thirty-five proteins were identified by matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS) analysis and protein database searching. Most of the identified proteins were associated with a variety of functions, including energy and metabolism (30%), programed cell death (9%), antioxidative processes (14%), chaperonin (23%), cell division, amino acid metabolism, secondary metabolism, and other functional classes. Furthermore, the expression patterns of HSP70 and cytosolic ascorbate peroxidase (cAPX) were validated by immunoblotting analysis. This study provides a novel, global insight into proteomic differences between normal and aborted seeds in longan. We anticipate that identification of the differentially expressed proteins may lead to a better understanding of the molecular basis for seed abortion in longan.

  • 相关文献

[1]Identification and analysis of differentially expressed proteins during cotyledon embryo stage in longan. Wang, Jing,Chen, Wei,Wang, Jing,Weng, Zhao-xia,Liu, Hao,Liang, Wen-yu,Chen, Wei,Cheng, Chi-Lien,Jiang, Ji-mou. 2010

[2]Proteomics Changes in Filling Seeds of Vegetable Soybean. Zhang, Yumei,Hu, Runfang,Li, Huawei,Zhu, Haisheng,Lin, Guoqiang,Zhao, Jinming,Guo, Na,Xing, Han.

[3]Analysis of 2-DE Profiles of Floral Reversion Bud Proteins in Longan. Chen, W.,Chen, W.,Long, Q.,Chen, Q. X.,Zheng, S. Q.. 2010

[4]Tentative identification of sex-specific antibodies and their application for screening bovine sperm proteins for sex-specificity. Yang, Wu-Cai,Yang, Wu-Cai,Xiao, Yao,Zhang, Hua-Lin,Tang, Ke-Qiong,Yang, Li-Guo,Sang, Lei.

[5]Peroxiredoxin IV Regulates Pro-Inflammatory Responses in Large Yellow Croaker (Pseudosciaena crocea) and Protects against Bacterial Challenge. Yu, Suhong,Mu, Yinnan,Ao, Jingqun,Chen, Xinhua,Yu, Suhong.

[6]The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Chen, Xiaodong,Jia, Aiqun,Tian, Dagang,Kong, Xiangxiang,Chen, Qian,Abd Allah, E. F.,Hu, Xiangyang.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台