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Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion

文献类型： 外文期刊

作者： Liu, Hao 1 ; Liu, Yan-zhen 1 ; Zheng, Shao-quan 2 ; Jiang, Ji-mou 2 ; Wang, Ping 3 ; Chen, Wei 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

2.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Hort Sci, Fuzhou 350002, Peoples R China

4.Fujian Agr & Forestry Univ, Key Lab, Minist Educ Genet, Fuzhou 350002, Peoples R China

关键词： Longan;Seed abortion;Proteomics;2-DE;MALDI-TOF-MS/MS

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

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收录情况： SCI

摘要： Two-dimensional gel electrophoresis (2-DE), coupled with mass spectroscopy, was used to study seed abortion in Dimocarpus longan Lour. (cv. Minjiao 64-1) by comparing normal and aborted seeds at three developmental stages. More than 1,000 protein spots were reproducibly detected in 2-DE gels, with 43 protein spots being significantly altered in their intensity between normal and aborted seeds at least at one stage. Thirty-five proteins were identified by matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS) analysis and protein database searching. Most of the identified proteins were associated with a variety of functions, including energy and metabolism (30%), programed cell death (9%), antioxidative processes (14%), chaperonin (23%), cell division, amino acid metabolism, secondary metabolism, and other functional classes. Furthermore, the expression patterns of HSP70 and cytosolic ascorbate peroxidase (cAPX) were validated by immunoblotting analysis. This study provides a novel, global insight into proteomic differences between normal and aborted seeds in longan. We anticipate that identification of the differentially expressed proteins may lead to a better understanding of the molecular basis for seed abortion in longan.

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