The transcription factor PbrbZIP52 positively affects pear pollen tube longevity by promoting callose synthesis
文献类型： 外文期刊
作者： Xia, Zhongheng 1 ; Wen, Binxu 1 ; Shao, Jing 1 ; Zhang, Tianci 1 ; Hu, Mengmeng 1 ; Lin, Lin 1 ; Zheng, Yiping 1 ; Shi, Zhixin 1 ; Dong, Xinlin 1 ; Song, Juanjuan 1 ; Li, Yuanshan 1 ; Wu, Yongjie 1 ; Yuan, Yafang 1 ; Wu, Juyou 1 ; Chen, Qingxi 1 ; Chen, Jianqing 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Peoples R China
2.Nanjing Agr Univ, Coll Hort, Ctr Pear Engn Technol Res, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
3.Jilin Acad Agr Sci, Inst Pomol, Gongzhuling 136100, Peoples R China
4.Fujian Agr & Forestry Univ, Anxi Coll Tea Sci, Anxi 362406, Peoples R China
5.Fujian Acad Agr Sci Biotechnol Inst, Fuzhou 350003, Peoples R China
6.Fujian Vocat Coll Agr, Dept Hort & Landscape Architecture 6, Fuzhou 350119, Peoples R China
期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）
ISSN： 0032-0889
年卷期： 2023 年 191 卷 3 期
页码：
收录情况： SCI
摘要： In pear (Pyrus bretschneideri), pollen tube growth is critical for the double fertilization associated with seed setting, which in turn affects fruit yield. The normal deposition of callose mediates the polar growth of pollen tubes. However, the mechanism regulating callose synthesis in pollen tubes remains relatively uncharacterized. In this study, we revealed that the typical pear pollen tube lifecycle has a semi-growth duration (GD(50)) of 16.16 h under in vitro culture conditions. Moreover, callose plugs were deposited throughout the pollen tube lifecycle. The formation of callose plugs was inhibited by 2-deoxy-D-glucose, which also accelerated the senescence of pear pollen tubes. Additionally, PbrCalS1B.1, which encodes a plasma membrane-localized callose synthase, was expressed specifically in pollen tubes and restored the fertility of the Arabidopsis (Arabidopsis thaliana) cals5 mutant, in which callose synthesis is inhibited. However, this restoration of fertility was impaired by the transient silencing of PbrCalS1B.1, which restricts callose plug formation and shortens the pear pollen tube lifecycle. More specifically, PbrbZIP52 regulated PbrCalS1B.1 transcription by binding to promoter A-box elements to maintain the periodic formation of callose plugs and normal pollen tube growth, ultimately leading to double fertilization. This study confirmed that PbrbZIP52 positively affects pear pollen tube longevity by promoting callose synthesis. This finding may be useful for breeding high-yielding pear cultivars and stabilizing fruit setting in commercial orchards.
- 相关文献
作者其他论文 更多>>
-
Integrated Transcriptomic and Metabolomic Analysis Revealed Regulatory Mechanisms on Flavonoids Biosynthesis in the Skin of Passion Fruit (Passiflora spp.)
作者：Fang, Ting;Zheng, Yiping;Ma, Qicheng;Ren, Rui;Xian, Huimin;Zeng, Lihui;Zheng, Yiping
关键词：passion fruit; flavonoids; metabolome; transcriptome; PeMYB114
-
Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia'
作者：Xu, Xiaoping;Yang, Chenglong;Zheng, Yiping;Guo, Wenjie;Fang, Shaozhong;Lai, Zhongxiong
关键词：riboflavin;
Lilium Oriental hybrid 'Siberia'; miRNA; transcriptomics; dormancy release -
Genetic diversity and comprehensive evaluation of physicochemical traits in Phyllanthus emblica L. for breeding and resource utilization
作者：Wang, Jianchao;Ali, Muhammad Moaaz;Wu, Yongjie;Zhang, Ge;Chen, Jianqing;Chen, Faxing;Wang, Jianchao;Zhang, Xiaoyan;Xie, Lixue;Zhang, Lijie;Li, Tao
关键词：Amla; Fruit quality; Diversity analysis; Tropical and subtropical fruit; Resource conservation
-
Establishment of an Efficient Agrobacterium rhizogenes-Mediated Hairy Root Transformation System for Functional Analysis in Passion Fruit
作者：Pan, Jiayi;Zheng, Yiping;Wang, Tiancai;Xiong, Pengpeng;Cui, Kaibo;Zeng, Lihui;Fang, Ting;Zheng, Yiping;Zeng, Lihui;Fang, Ting
关键词：passion fruit;
Agrobacterium rhizogenes ; hairy root;PeMYB123 ; proanthocyanidin -
Rapid detection of pigeon adenovirus 2 using a TaqMan real-time PCR assay
作者：Chen, Cuiteng;Zhu, Chunhua;Chen, Zhen;Cai, Guozhang;Lin, Lin;Zhang, Shizhong;Jiang, Bin;Miao, Zhongwei;Fu, Guanghua;Huang, Yu;Wan, Chunhe
关键词：pigeon adenovirus 2; fiber 2; TaqMan-qPCR assay; YPDS; epidemiological surveillance
-
Characterisation and Expression Analysis of LdSERK1, a Somatic Embryogenesis Gene in Lilium davidii var. unicolor
作者：Wang, Shaojuan;Yi, Xiaoyan;Zhang, Lijuan;Ali, Muhammad Moaaz;Ke, Mingli;Lu, Yuxian;Chen, Faxing;Zheng, Yiping;Cai, Xuanmei;Fang, Shaozhong;Lin, Zhimin;Wu, Jian
关键词：gene isolation; somatic embryogenesis receptor-like kinase (SERK) gene; LdSERK1; phenotyping; Lilium davidii var. unicolor
-
Novel R2R3-MYB Transcription Factor LhMYB1 Promotes Anthocyanin Accumulation in Lilium concolor var. pulchellum
作者：Tian, Shengnan;Ali, Muhammad Moaaz;Ke, Mingli;Lu, Yuxian;Chen, Faxing;Zheng, Yiping;Cai, Xuanmei;Fang, Shaozhong;Lin, Zhimin;Wu, Jian
关键词：transcription factor; LhMYB1; anthocyanin accumulation; Lilium concolor var. pulchellum
