Development and characterization of cell lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicus
文献类型： 外文期刊
作者： Ye, H. -Q. 1 ; Chen, S. -L. 2 ; Sha, Z. -X. 2 ; Xu, M. -Y. 3 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China; Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; Shanghai Fisheries Univ, Shanghai Univ E Inst, Aquaculture Div, Shanghai 200090, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China; Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R Chin
期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：2.051； 五年影响因子：2.25 ）
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收录情况： SCI
摘要： Five cell lines (LJHK, LJS, LJL, LJH-1 and LJH-2) were established from the head kidney, spleen, liver and heart of sea perch Lateolabrax japonicus. The cell lines LJHK, LJS, LJL, LJH-1 and LJH-2 were subcultured 46, 32, 32, 36 and 34 times in minimumessential medium (MEM) supplemented with foetal bovine serum (FBS), sea perch serum and 10 ng ml-1 basic fibroblast growth factor (bFGF). Morphology of primary cultures and subcultures of the five cell lines were observed continuously by microscopy. Thesuitable temperature for growth was 18 to 30o C for all of these cell lines with the optimum growth at 24o C and a reduced growth rate <18o C. The optimum concentration of FBS was found to be 10% and addition of bFGF to the medium significantly increased the growth rate of the cells. The doubling time of LJS, LJH-1, LJL, LJH-2 and LJHK cells was determined to be 52.7, 54.9, 57, 58.7 and 66 h at a plating density of 1 x 105 cells ml-1 at 24o C, respectively. Chromosome analysis revealed that 42, 48, 38,43 and 45% cells maintained normal diploid chromosome number (48) in the LJH-1, LJH-2, LJHK, LJL and LJS cell lines, respectively. The LJHK cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids and the expression ofGFP gene in the cells indicated the possible utility of the cells in gene expression studies. Furthermore, treatment of the LJHK cells with lipopolysaccharide led to increased expression of IL-1beta, demonstrating that LJHK cells might be a valuable tool for studying the expression and function of immunomodulatory gene in fishes.
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