Highly resistant and sensitive colorimetric immunochromatographic assay for sibutramine (SBT) illegally adulterated into diet food based on PDA/AuNP labelling
文献类型： 外文期刊
作者： Zhao, Yun 1 ; Huang, Sijie 4 ; Chao, Mengjia 1 ; Wang, Yulong 2 ; Liu, Pengyan 2 ; Li, Pan 2 ; Fang, Xuechen 5 ; Routledge, Michael N. 6 ; Peng, Chifang 1 ; Zhang, Cunzheng 2 ;
作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, PR, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual, Nanjing 210014, PR, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Safety State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing 210014, PR, Peoples R China
4.Nanjing Inst Environm Sci, Minist Ecol & Environm, Nanjing 210042, PR, Peoples R China
5.Nanchang Univ, Coll Food Sci & Technol, Nanchang 330031, PR, Peoples R China
6.Univ Leeds, Sch Med, Leeds LS2 9JT, W Yorkshire, England
7.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Jiangsu, Peoples R China
8.Jiangsu Univ, Int Joint Res Lab Intelligent Agr & Agri Prod Pro, Jiangsu Educ Dept, Zhenjiang, Peoples R China
期刊名称：ANALYST （ 影响因子：4.2； 五年影响因子：4.1 ）
ISSN： 0003-2654
年卷期： 2023 年 148 卷 20 期
页码：
收录情况： SCI
摘要： A gold nanoparticle (AuNP) based immunochromatographic assay strip is a valuable tool for monitoring chemicals in foods. However, the sensitive ICA strip for SBT is rarely reported due to the fact that monoclonal antibodies (mAbs) against SBT with high affinity are commercially unavailable. Herein, a monoclonal antibody against SBT was prepared through a designed hapten with a carboxyl end-capped space arm. The obtained mAb showed high affinity for SBT and N-desmethylsibutramine, a metabolite of SBT. Furthermore, a series of core-shell NPs, polydopamine (PDA) coated AuNPs (PDA/AuNPs) with controlled shell thickness and packing density were synthesized. The obtained PDA/AuNP-mAb conjugate demonstrated high tolerance to salt and good stability in a wide pH range, which is beneficial for improving the matrix interference common in ICA. As a result, PDA/AuNP-based ICA could quantify SBT in the range of 3.39-69.60 ng mL-1 with a limit of detection (LOD) of 0.98 ng mL-1. This novel ICA improved the sensitivity of the traditional AuNP-based ICA by nearly 12 times. Method validation was conducted with spiked samples of slimming food and human serum and compared with HPLC-MS/MS. Consistent results indicated that high sensitivity, accuracy, and reliability of the PDA/AuNP-based ICA approach were achieved. To the best of our knowledge, this study reported the most sensitive immunoassay for SBT thus far. Polydopamine coated AuNPs (PDA/AuNPs) with controlled shell thickness and packing density are synthesized and conjugated to mAb, which demonstrate high tolerance to salt and good stability in a wide pH range and improve the sensitivity of ICA.
- 相关文献
作者其他论文 更多>>
-
Quantitative determination of prothioconazole in wheat grain, soybean, and pond water based on a polyclonal antibody
作者：Guo, Zhihui;Fang, Qingkui;Liao, Min;Fang, Qingkui;Guo, Zhihui;Deng, Mingya;Fang, Wenwen;Zheng, Kang;Liao, Min;Fang, Qingkui;Wang, Yulong;Wang, Yulong
关键词：
-
Fluorescent and Colorimetric Dual-Readout Immunochromatographic Assay for the Detection of Phenamacril Residues in Agricultural Products
作者：Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Jiang, Lan;Zhang, Cunzheng;Zhao, Yun;Xue, Feng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：fluorescent and colorimetric dual-readout; immunochromatographicassay; phenamacril; monoclonal antibody; polydopamine coated gold nanoparticles; quantum dots
-
Preparation of monoclonal antibody and establishment of indirect competitive chemiluminescence enzyme immunoassay for sodium pentachlorophenolate
作者：Dong, Sa;Shi, Qiuyun;Guan, Lingjun;Feng, Jianguo;Guan, Lingjun;Wang, Yulong;Liu, Pengyan;Zhang, Cunzheng
关键词：Sodium pentachlorophenate; Monoclonal antibody; ic-CLEIA; Residual detection; Animal tissues
-
Colorimetric and Reverse Fluorescence Dual-Signal Readout Immunochromatographic Assay for the Sensitive Determination of Sibutramine
作者：Gui, Yun;Zhang, Cunzheng;Gui, Yun;Zhao, Yun;Liu, Pengyan;Wang, Yulong;Mao, Xinxin;Zhang, Cunzheng;Zhao, Yun;Peng, Chifang;Mao, Xinxin;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：
-
Competitive ratiometric fluorescent lateral flow immunoassay based on dual emission signal for sensitive detection of chlorothalonil
作者：Fang, Xuechen;Liu, Tingting;Xue, Guangjian;Wu, Mengyun;Lai, Weihua;Peng, Juan;Fang, Xuechen;Liu, Pengyan;Zhang, Cunzheng;Xue, Chang;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：Fluorescent magnetic nanobeads; Aggregation-induced emission; Immunomagnetic separation; Lateral flow immunoassay; Ratiometric fluorescence
-
Uncovering the Nutritive Profiles of Adult Male Chinese Mitten Crab (E. sinensis) Harvested from the Pond and Natural Water Area of Qin Lake Based on Metabolomics
作者：Ye, Yuhui;Wang, Yulong;Liu, Pengyan;Chen, Jian;Zhang, Cunzheng
关键词：harvest time; metabolomic profiling; polyunsaturated fatty acid; pond culture
-
Highly sensitive fluorescent turn-on lateral flow strip for chlorothalonil based on an indicator displacement ratiometric fluorescent assay
作者：Ren, Hongxin;Li, Guowen;Yue, Xin;Wang, Zhouping;Peng, Chifang;Ren, Hongxin;Li, Guowen;Yue, Xin;Wang, Zhouping;Peng, Chifang;Chen, Xiujin;Peng, Chifang;Wang, Zhouping;Peng, Chifang
关键词：Indicator displacement assays; Chlorothalonil sensing; Dual signal responses; Ratio fluorometric; Lateral flow assay
