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Transcription Factor and Protein Regulatory Network of PmACRE1 in Pinus massoniana Response to Pine Wilt Nematode Infection

文献类型： 外文期刊

作者： Xie, Wanfeng 1 ; Lai, Xiaolin 2 ; Wu, Yuxiao 2 ; Li, Zheyu 1 ; Zhu, Jingwen 2 ; Huang, Yu 4 ; Zhang, Feiping 2 ;

作者机构： 1.Fujian Agr & Forestry Univ, Jinshan Coll, Fuzhou 350002, Peoples R China

2.Fujian Agr & Forestry Univ, Key Lab Integrated Pest Management Ecol Forests Fu, Fuzhou 350002, Peoples R China

3.Fujian Agr & Forestry Univ, Forestry Coll, Fuzhou 350002, Peoples R China

4.Fujian Acad Agr Sci, Fuzhou 350000, Peoples R China

关键词： masson pine; pine wilt disease; PmACRE1; transcription factor; protein-protein interactions

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 19 期

页码：

收录情况： SCI

摘要： Pine wilt disease, caused by Bursaphelenchus xylophilus, is a highly destructive and contagious forest affliction. Often termed the "cancer" of pine trees, it severely impacts the growth of Masson pine (Pinus massoniana). Previous studies have demonstrated that ectopic expression of the PmACRE1 gene from P. massoniana in Arabidopsis thaliana notably enhances resistance to pine wilt nematode infection. To further elucidate the transcriptional regulation and protein interactions of the PmACRE1 in P. massoniana in response to pine wilt nematode infection, we cloned a 1984 bp promoter fragment of the PmACRE1 gene, a transient expression vector was constructed by fusing this promoter with the reporter GFP gene, which successfully activated the GFP expression. DNA pull-down assays identified PmMYB8 as a trans-acting factor regulating PmACRE1 gene expression. Subsequently, we found that the PmACRE1 protein interacts with several proteins, including the ATP synthase CF1 alpha subunit, ATP synthase CF1 beta subunit, extracellular calcium-sensing receptor (PmCAS), caffeoyl-CoA 3-O-methyltransferase (PmCCoAOMT), glutathione peroxidase, NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase 1, cinnamyl alcohol dehydrogenase, auxin response factor 16, and dehydrin 1 protein. Bimolecular fluorescence complementation (BiFC) assays confirmed the interactions between PmACRE1 and PmCCoAOMT, as well as PmCAS proteins in vitro. These findings provide preliminary insights into the regulatory role of PmACRE1 in P. massoniana's defense against pine wilt nematode infection.

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