Isolation and characterization of a novel glycogen synthase kinase-3 gene, GmGSK, in Glycine max L. that enhances abiotic stress tolerance in Saccharomyces cerevisiae
文献类型: 外文期刊
作者: Zhang, Chunbao 1 ; Zhao, Hongkun 2 ; Liu, Yanzhi 2 ; Li, Qiyun 2 ; Liu, Xiaodong 2 ; Tan, Hua 2 ; Yuan, Cuiping 2 ; Dong, 1 ;
作者机构: 1.NE Normal Univ, Coll Life Sci, Changchun 130024, Peoples R China
2.Jilin Acad Agr Sci, Biotechnol Res Ctr, Changchun 130033, Peoples R China
关键词: abiotic stress tolerance enhancement
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
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收录情况: SCI
摘要: A novel glycogen synthase kinase-3 gene, GmGSK, was isolated from Glycine max. It is 1,596 bp in length with one ORF of 410 amino acids. Southern blot analysis revealed that it has at least two copies in the G. max genome. GmGSK, when transiently expressed in Nicotiana tabacum leaves, was localized in both cell membrane and cytoplasm. Northern blot analysis indicated that GmGSK is expressed in all tissues, with highest expression in the root. GmGSK can be induced by various abiotic stresses. When transformed with GmGSK, Saccharomyces cerevisiae exhibited enhanced resistance to salt and drought stress.
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