Interaction of Xestia c-nigrum granulovirus with peritrophic matrix and Spodoptera litura nucleopolyhedrovirus in Spodoptera litura
文献类型： 外文期刊
作者： Guo, H. F. 1 ; Fang, J. C. 1 ; Wang, J. P. 1 ; Zhong, W. F. 1 ; Liu, B. S. 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
关键词： Xestia c-nigrum granulovirus;Spodoptera litura nucleopolyhedrovirus;peritrophic matrix;Spodoptera litura;NUCLEAR POLYHEDROSIS-VIRUS;VIRAL ENHANCING FACTOR;NI GRANULOSIS-VIRUS;PSEUDALETIA-UNIPUNCTA;BACULOVIRUS ENHANCIN;SYNERGISTIC FACTOR;MEMBRANE;LEPIDOPTERA;ENHANCEMENT;INFECTION
期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）
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收录情况： SCI
摘要： Xestia c-nigrum granulovirus (XcGV) was tested for its ability to increase Spodoptera litura nucleopolybedrovirus (SINPV) infection in larvae of S. litura (F.). The interaction of XcGV with peritrophic matrix and SINPV in S. litura also was studied to account for the synergism. In dose-response bioassays with a constant XcGV concentration of 5-mg/ ml capsules and SINPV concentration that varied from 10(3) to 10(7) polyhedral inclusion bodies (PIB) per larva, XcGV increased the virulence of SINPV infection in fifth instars of S. litura. The lethal concentration of 50% individuals (LC50) of SINPV combined with XcGV was 3.35 X 10(5)PIB/ml, which was significantly lower than that of SINPV alone (2.17 X 10(6)). Compared with 10(7) PIB/ml SINPV alone, the lethal time of 50% individuals (LT50) of 10(7) PIB/ml SINPV combined with XcGV was not significantly shortened. In addition, no significant improvement in the activity and killing speed of SINPV progeny was noted after propagation with XcGV, indicating that native characters of SINPV associated with viral potency were not altered by XcGV. Investigation via environmental scanning electronic microscopy showed that the peritrophic matrix (PM) of S. litura exposed to XcGV or XcGV enhancin, or the combination treatment, was markedly disrupted. The outer surface of the PM was loose, or ruptured, which potentially facilitated the passage of virions through the PM. Therefore, it is reasonable to conclude that the synergy between XcGV and SINPV was closely associated with the disruption of the PM in S. litura.
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