Biotin-labelled peptidomimetic for competitive time-resolved fluoroimmunoassay of benzothiostrobin
文献类型： 外文期刊
作者： Ding, Yuan 1 ; Chen, He 1 ; Zong, Lingfeng 1 ; Cui, Panpan 1 ; Wu, Xujin 2 ; Wang, Minghua 1 ; Hua, Xiude 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
2.Henan Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Zhengzhou 450002, Peoples R China
关键词： Peptidomimetic; Phage display; Pesticide residue; Time-resolved fluoroimmunoassay
期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.478； 五年影响因子：4.29 ）
ISSN： 1618-2642
年卷期：
页码：
收录情况： SCI
摘要： In recent years, more and more functional peptide ligands have been identified from phage display libraries and served the immunoassay of small molecules. After the identification, the phage particle instead limits further application of peptide ligands, so it is of great significance to explore the peptide ligand as an independent detection reagent. In this work, the identified peptidomimetic of benzothiostrobin was synthesized and labelled with biotin, which was combined with Eu3+-labelled streptavidin to develop the peptide-based time-resolved fluoroimmunoassay (P-TRFIA). Under the optimal conditions, the half-maximum inhibitory concentration (IC50) of proposed P-TRFIA is 3.63 ng mL(-1), which is similar to the TRFIA using phage-borne peptidomimetic and Eu-3(+)-labelled anti-phage antibody (IC50: 4.55 ng mL(-1)), also more sensitive than previously reported immunoassays for benzothiostrobin. In addition, the proposed P-TRFIA shows excellent specificity and accuracy for analysis of spiked samples, and its detection results shows good consistency with high-performance liquid chromatography for the detection of environment and agro-products samples with unknown benzothiostrobin concentrations.
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