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Transient virus-induced gene silencing of MaBAM9b efficiently suppressed starch degradation during postharvest banana fruit ripening

文献类型： 外文期刊

作者： Liu, Mengting 1 ; Li, Meng 2 ; Wang, Yudi 1 ; Wang, Jingyi 2 ; Miao, Hongxia 2 ; Wang, Zhuo 2 ; Xu, Biyu 2 ; Li, Xinguo 1 ;

作者机构： 1.Hainan Univ, Coll Hort, Haikou 571100, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Minist Agr, 4 Xueyuan Rd, Haikou 571101, Hainan, Peoples R China

3.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China

4.Chinese Acad Trop Agr Sci, Hainan Acad Trop Agr Resource, Hainan Key Lab Protect & Utilizat Trop Bioresourc, 4 Xueyuan Rd, Haikou 571101, Hainan, Peoples R China

关键词： Banana (Musa acuminata AAA group cv Brazilian); Virus-induced gene silencing (VIGS); MaBAM9b; Functional identification

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）

ISSN： 1863-5466

年卷期： 2021 年 15 卷 4 期

页码：

收录情况： SCI

摘要： The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be improved. The banana is a typical starch conversion fruit, and beta-amylase is a key enzyme that may play an important role in starch degradation during the ripening process. Musa acuminata beta-amylase 9b (MaBAM9b) is closely related to starch degradation. However, its exact function in starch degradation has not been demonstrated in banana. Stable genetic transformation to identify gene function is a time- and energy-consuming process. Thus, an efficient and rapid method is needed for functional identification. Virus-induced gene silencing (VIGS) is a reverse-genetics method based on RNA-mediated antiviral plant defense that has been used to rapidly identify gene functions in plants. The aim of this study was to optimize a transient VIGS system and functionally elucidate MaBAM9b in postharvest banana fruit. Using 2- to 4-mm-thick fruit slices, vacuum infiltration of suspensions of Agrobacterium strains carrying TRV1 and TRV2-MaBAM9b, 0.5% iodine-potassium-iodide (I-2-KI) staining for 150 s, and 1:3 TRV1:TRV2-MaBAM9b cultivation at 30 mmHg for 30 s achieved an optical density (OD) of 0.8 at 600 nm; after being incubated on Murashige and Skoog (MS) media for 5 days (d), starch degradation was efficiently suppressed during postharvest banana fruit ripening, as determined by I2-KI staining, total starch content, beta-amylase activity, soluble sugar content, and endogenous MaBAM9b expression. The system described here is particularly useful for studying genes and networks involved in starch conversion in fruits, which alone would not produce a visual phenotype. This system will provide a platform for functional genomics and fruit quality improvement in banana.

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