Effect of titanium dioxide nanoparticle (TiO2-NP) exposure in a novel Amur sturgeon Acipenser schrenckii hepatocyte cell line
文献类型： 外文期刊
作者： Zhou, Zhou 1 ; Liu, Ting 1 ; Luo, Tianxun 1 ; Zhao, Zhenxing 1 ; Zhu, Junquan 3 ;
作者机构： 1.Guizhou Acad Agr Sci, Guizhou Fisheries Res Inst, Guiyang, Peoples R China
2.Guizhou Special Aquat Prod Engn Technol Ctr, Guiyang, Peoples R China
3.Ningbo Univ, Key Lab Aquacultural Biotechnol, Minist Educ, Ningbo, Peoples R China
4.Ningbo Univ, Coll Marine Sci, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo, Peoples R China
关键词： Acipenser schrenckii; apoptosis; cell line; cytotoxicity; titanium dioxide nanoparticle
期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：1.7； 五年影响因子：2.1 ）
ISSN： 0022-1112
年卷期： 2024 年
页码：
收录情况： SCI
摘要： In vitro cell culture is crucial for predicting the toxicity of titanium dioxide nanoparticle (TiO2-NP). However, assessing the toxicity of TiO2-NPs in sturgeon remains difficult given the lack of sufficient cell lines. We established and characterized the first hepatocyte cell line from Acipenser schrenckii liver tissue (ASL). This ASL cell line proliferated well in Dulbecco's modified Eagle's medium at 25 degrees C and 10% fetal bovine serum. ASL cells with a chromosome number of 244 were successfully transfected with the pEGFP-N3 plasmid. The ASL cell line's origin was verified as A. schrenckii through mitochondrial cytochrome C oxidase I and mitochondrial 16S ribosomal RNA (rRNA) sequencing. Using the ASL cell line as an in vitro model, we found that TiO2-NP exposure decreased the viability and promoted the damage of ASL cells (96-h LC50 = 331.8 mu g mL(-1)). Increased reactive oxygen species and malondialdehyde levels in ASL cells suggested oxidative stress under TiO2-NP exposure. We also observed dysregulation of aspartate aminotransferase and alanine aminotransferase levels. By detecting calcium ions and mitochondrial membrane potential indicators, we found that the apoptotic pathway induced by endoplasmic reticulum stress played a major role at low concentrations of TiO2-NP-induced stress. Both mitochondria-mediated and endoplasmic reticulum stress promoted apoptosis under increasing TiO2-NP concentrations. In conclusion, the ASL cell line established in this study is a useful in vitro model for toxicological studies of TiO2-NP exposure in fish.
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