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Identification of a Proteolytic Bacterium, HW08, and Characterization of Its Extracellular Cold-Active Alkaline Metalloprotease Ps5

文献类型： 外文期刊

作者： Yang, Chengye 1 ; Wang, Fang 1 ; Hao, Jianhua 1 ; Zhang, Kai 3 ; Yuan, Na 3 ; Sun, Mi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

2.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China

3.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China

关键词： Pseudomonas lundensis;psychrophilic;metalloprotease;characterization;industrial application

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）

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收录情况： SCI

摘要： A psychrophilic protease-producing bacterium, HW08, was isolated from sediment of the Yellow Sea in eastern China. On the basis of 16S rDNA sequence analysis and physiological properties, the isolate was identified as Pseudomonas lundensis. The secreted protease, named Ps5, was purified from the culture supernatant as a monomer with an apparent molecular mass of 46kDa on SDS-PAGE. As a metalloprotease (inhibited by EDTA), the enzyme showed maximum activity at 30 °C at pH 10.4. It had no activity loss exposed at 4°C for 60 d or under repeated freezing and thawing. Broad temperature (25-40 °C) and pH (7.0-11.0) stability was observed in the presence of 5 mm Ca~(2+). Furthermore, the enzyme was resistant to detergent additives such as non-ionic surfactants and bleaches. It showed considerable potential for industry that requires alkaline-protease.

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