Haploid induction and plant production in bottle gourd by pollination with gamma irradiated pollen
文献类型： 外文期刊
作者： Zhao, Qing 1 ; Zhang, Man 1 ; Qi, Han 1 ; Yang, Xingping 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing, Jiangsu, Peoples R China
2.Jiangsu Vocat Coll Agr & Forestry, Dept Agron & Hort, Zhenjiang, Jiangsu, Peoples R China
3.Jiangsu Univ, Sch Life Sci, Zhenjiang, Jiangsu, Peoples R China
关键词： Bottle gourd; Haploid; Irradiation; Parthenogenesis
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：3.0； 五年影响因子：3.0 ）
ISSN： 0167-6857
年卷期： 2022 年
页码：
收录情况： SCI
摘要： Bottle gourd [Lagenaria siceraria (Molina) Standley] has been used exclusively as rootstocks for Cucurbit crops. Haploid technique increases the selection efficiency for developing resistant bottle gourd varieties. This work focused on haploid induction in bottle gourd through in situ parthenogenesis by pollination with gamma-irradiated pollen. Pollination were carried out for six genotypes of bottle gourd with 50, 75, 100, 125 and 150 Gy (Gy) doses of gamma rays. Production of in vitro haploid plants was influenced by irradiation dose, genotype and embyo stage. Seeds at 17 to 21 days after pollination was found to be optimum for embryo rescue. Pollination with irradiated pollen at 100 to 125 Gy was effective for haploid induction. The ploidy level of the 11 parthenogenetic plantlets was confirmed by flow cytometry and 36.3% haploid and 9% triploid plantlets were obtained. This work provides valuable germplasm resources for bottle gourd genetic analysis and breeding programs.
- 相关文献
作者其他论文 更多>>
-
Cucumber Auxin Response Factor CsARF10a Regulates Leaf Morphogenesis and Parthenocarpic Fruit Set in Tomato
作者：Xu, Jian;Yao, Xiefeng;Lou, Lina;Zhang, Man;Liu, Guang;Yang, Xingping;Liu, Jinqiu;Zhu, Lingli;Hou, Qian;Xu, Jinhua;Zhu, Pinyu;Meng, Yongjiao;Li, Ji
关键词：ARFs; cucumber; CsARF10a; parthenocarpic fruit set
-
A Transcriptomic Analysis of Bottle Gourd-Type Rootstock Roots Identifies Novel Transcription Factors Responsive to Low Root Zone Temperature Stress
作者：Liu, Jinqiu;Zhang, Man;Xu, Jian;Yao, Xiefeng;Lou, Lina;Hou, Qian;Zhu, Lingli;Yang, Xingping;Liu, Guang;Xu, Jinhua;Liu, Jinqiu;Zhang, Man;Xu, Jian;Yao, Xiefeng;Lou, Lina;Hou, Qian;Zhu, Lingli;Yang, Xingping;Liu, Guang;Xu, Jinhua
关键词：bottle gourd; root; low root zone temperature; RNA-Seq; transcription factor
-
Comparative metabolomics profiling reveals the molecular information of whole and fresh-cut melon fruit (cv. Xizhoumi-17) during storage
作者：Wu, Zhangfei;Yu, Zhifang;Wu, Zhangfei;Shi, Zhenxia;Xie, Chunyan;Wu, Zhangfei;Yang, Xingping;Xu, Jinhua
关键词：Melon fruit; Postharvest; Cutting; Metabolomics; LC-MS
-
Genome-wide identification and analysis of promising GDSL-type lipases related to gummy stem blight resistance in watermelon (Citrullus lanatus)
作者：Ren, Runsheng;Yang, Xingping;Xu, Jinhua;Zhang, Man;Liu, Guang;Yao, Xiefeng;Lou, Lina;Xu, Jian;Zhu, Lingli;Hou, Qian;Ren, Runsheng;Zhang, Keyun
关键词：Citrullus lanatus; GDSL family; Gummy stem blight; Phylogenetic analysis; Genome-wide identification
-
Characterization of Fusarium root rot disease in grafted watermelon
作者：Zhang, Man;Yang, Xingping;Xu, Jinhua;Liu, Guang;Yao, Xiefeng;Ren, Runsheng
关键词：Fusarium oxysporum; Grafted watermelon; Resistance; Rootstock; Root rot
-
Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings
作者：Zhang, Man;Xu, Jinhua;Ren, Runsheng;Liu, Guang;Yao, Xiefeng;Lou, Lina;Xu, Jian;Yang, Xingping;Zhang, Man;Ren, Runsheng
关键词：bottle gourd; Citrullus lanatus; Fusarium oxysporum f; sp; niveum; proteomics; rootstock grafting
-
Effect of cutting and storage temperature on sucrose and organic acids metabolism in postharvest melon fruit
作者：Wu, Zhangfei;Tu, Mingmei;Yu, Zhifang;Yang, Xingping;Xu, Jinhua
关键词：Fresh-cut melon; Sucrose; Organic acid; Metabolism; Temperature
