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Preparation of monoclonal antibody and establishment of indirect competitive chemiluminescence enzyme immunoassay for sodium pentachlorophenolate

文献类型： 外文期刊

作者： Dong, Sa 1 ; Shi, Qiuyun 1 ; Guan, Lingjun 1 ; Wang, Yulong 2 ; Liu, Pengyan 2 ; Zhang, Cunzheng 2 ; Feng, Jianguo 1 ;

作者机构： 1.Yangzhou Univ, Coll Plant Protect, Yangzhou 225009, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, Key Lab Control Technol & Stand Agroprod Safety &, State Key Lab Breeding Base,Minist Agr,Inst Food S, Nanjing 210014, Peoples R China

关键词： Sodium pentachlorophenate; Monoclonal antibody; ic-CLEIA; Residual detection; Animal tissues

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.8； 五年影响因子：4.5 ）

ISSN： 0026-265X

年卷期： 2024 年 196 卷

页码：

收录情况： SCI

摘要： In this study, three haptens of sodium pentachlorophenolate (PCP-Na) were designed and synthesized, and the monoclonal antibody against PCP-Na with high specificity and affinity was successfully prepared. Based on this monoclonal antibody, a highly sensitive indirect competitive chemiluminescence enzyme immunoassay (icCLEIA) was developed for the determination of PCP-Na and its metabolite pentachlorophenol (PCP). The icCLEIA method based on optimal conditions showed that the half maximal inhibitory concentration (IC50), linear range (IC20-IC80) and the detection limit (LOD, IC10) for PCP-Na were 0.083 ng/mL, 0.0076-1.5111 ng/ mL, and 0.0032 ng/mL, respectively, and 0.29 ng/mL, 0.0983-0.7008 ng/mL, and 0.0192 ng/mL for PCP, respectively. The results of cross reaction showed that the method had no obvious cross reaction with other six chlorophenols compounds (phenol, 2-chlorophenol, 4-chlorophenol, 2, 4-dichlorophenol, 2, 4, 5-trichlorophenol, 2, 4, 6-trichlorophenol) except the metabolite PCP. The average recoveries in chicken, pork and fish samples were 81.3-105.8 %, with coefficient of variation (CV) lower than 7.6 %. In this study, a new highly rapid, sensitive and accurate ic-CLEIA method was established, which can be used for the sensitive detection of PCP-Na and its metabolite PCP in animal tissues.

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