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BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa

文献类型： 外文期刊

作者： Su, Tongbing 1 ; Wang, Weihong 1 ; Wang, Zheng 1 ; Li, Peirong 1 ; Xin, Xiaoyun 1 ; Yu, Yangjun 1 ; Zhang, Deshuang 1 ; Zhao, Xiuyun 1 ; Wang, Jiao 1 ; Sun, Liling 1 ; Jin, Guihua 1 ; Zhang, Fenglan 1 ; Yu, Shuancang 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, State Key Lab Vegetable Biobreeding, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

3.Beijing Key Lab Vegtable Germplasms Improvement, Beijing 100097, Peoples R China

4.Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

期刊名称：CELL REPORTS （ 2022影响因子：8.8； 五年影响因子：9.9 ）

ISSN： 2211-1247

年卷期： 2023 年 42 卷 8 期

页码：

收录情况： SCI

摘要： Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a genome-wide association study in a natural population and quantitative trait locus mapping in an F2 population and identify that the MYB transcription factor BrMYB108 regulates plant resistance to VW. A 179 bp insertion in the BrMYB108 promoter alters its expression pattern during Verticillium longisporum (VL) infection. High BrMYB108 expression leads to high VL resistance, which is confirmed by disease resistance tests using BrMYB108 overexpression and loss-of-function mutants. Furthermore, we verify that BrMYB108 confers VL resistance by regulating reactive oxygen species (ROS) generation through binding to the promoters of respiratory burst oxidase genes (Rboh). A loss-of-function mutant of AtRbohF in Arabidopsis shows significant susceptibility to VL. Thus, BrMYB108 and its target ROS genes could be used as targets for genetic engineering for VL resistance of B. rapa.

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