文献类型： 外文期刊

作者： Ji, Lin ¹ ; Lin, Xisha ² ; Yuan, Kenan ¹ ; Li, Yue ¹ ; Leghari, Ambreen ¹ ; Yuan, Bingbing ⁵ ; Lin, Huixing ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Joint Int Res Lab Anim Hlth & Food Safety, MOE, Nanjing 210095, Peoples R China

2.Chuzhou Univ, Sch Biol Sci & Food Engn, Chuzhou 239000, Peoples R China

3.Nanjing Agr Univ, Coll Food Sci & Technol, Jiangsu Collaborat Innovat Ctr Meat Prod Proc & Qu, Nanjing 210095, Peoples R China

4.Shaheed Benazir Bhutto Univ Vet & Anim Sci Sakrand, Sindh 67210, Pakistan

5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： Swinepox virus; Salmonella; sseB; Vaccine

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.848； 五年影响因子：3.957 ）

ISSN： 0882-4010

年卷期： 2022 年 172 卷

页码：

收录情况： SCI

摘要： Salmonella spp. poses a great threat to the livestock, food safety and public health. A recombinant swinepox virus expressing a protective antigen sseB was constructed by homologous recombination to develop a vaccine against Salmonella infection. The rSPV-sseB was verified using PCR, Western blot and indirect immunofluorescence assay. The immune responses and protective efficacy of rSPV-sseB were assessed in piglets. Forty piglets were immunized with rSPV-sseB, inactive Salmonella vaccine, wild-type SPV (wtSPV), or PBS. The results showed that the level of the sseB-specific antibody of the rSPV-sseB-vaccinated piglets was significantly higher at all time points post-vaccination than those of the inactivated Salmonella vaccine (P < 0.05), wtSPV (P < 0.001) or mock treated piglets (P < 0.001). The IL-4 and IFN-gamma in the rSPV-sseB group were significantly higher than the other three groups at all post-infection time points. rSPV-sseB provided piglets with strong protection against the challenge of S. typhimurium with lethal dose. These results suggest the possibility of using recombinant swinepox virus rSPV-sseB as a promising vaccine to prevent Salmonella infection.