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Key Contributions of the Overexpressed Plutella xylostella Sigma Glutathione S-Transferase 1 Gene (PxGSTs1) in the Resistance Evolution to Multiple Insecticides

文献类型： 外文期刊

作者： Liu, Jiyuan 1 ; Tian, Zhen 1 ; Li, Ruichi 1 ; Ni, Shujun 1 ; Sun, Hong 1 ; Yin, Fei 2 ; Li, Zhenyu 2 ; Zhang, Yalin 1 ; Li, Yifan 1 ;

作者机构： 1.Northwest A&F Univ, Coll Plant Protect, Key Lab Plant Protect Resources & Pest Management, Minist Educ,Entomol Museum, Yangling 712100, Shaanxi, Peoples R China

2.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou 510640, Guangdong, Peoples R China

关键词： adaptive evolution; insecticide resistance; glutathione S-transferase; Plutella xylostella; microRNA

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 5 期

页码：

收录情况： SCI

摘要： The overexpression of insect detoxification enzymes is a typical adaptive evolutionary strategy for insects to cope with insecticide pressure. In this study, we identified a glutathione S-transferase (GST) gene, PxGSTs1, that exhibited pronounced expression in the field-resistant population of Plutella xylostella. By using RNAi (RNA interference), the transgenic fly models, and quantitative real-time polymerase chain reaction (RT-qPCR) methods, we confirmed that the augmented expression of PxGSTs1 mediates the resistance of P. xylostella to various types of insecticides, including chlorantraniliprole, novaluron, lambda-cyhalothrin, and abamectin. PxGSTs1 was found to bolster insecticide resistance in two ways: direct detoxification and enhancing antioxidative defenses. In addition, our findings demonstrated that pxy-miR-8528a exerts a pivotal influence on forming insecticide resistance in P. xylostella by downregulating PxGSTs1 expression. In summary, we elucidated the multifaceted molecular and biochemical underpinnings of PxGSTs1-driven insecticide resistance in P. xylostella. Our results provide a new perspective for understanding the insecticide resistance mechanism of P. xylostella.

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