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Identification of a novel laccase gene EuLAC1 and its potential resistance against Botrytis cinerea

文献类型： 外文期刊

作者： Zhao, Yichen 1 ; Liu, Yuqian 1 ; Dong, Xuan 1 ; Liu, Jia-Jia 1 ; Zhao, De-Gang 1 ;

作者机构： 1.Guizhou Univ, Coll Tea Sci, Key Lab Plant Resources Conservat & Germplasm Inn, Coll Life Sci,Minist Educ, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Guiyang 550006, Peoples R China

关键词： Laccase; EuLAC1; Transgenic tobacco; Lignin; Gray mold

期刊名称：TRANSGENIC RESEARCH （ 影响因子：3.145； 五年影响因子：2.71 ）

ISSN： 0962-8819

年卷期： 2022 年 31 卷 2 期

页码：

收录情况： SCI

摘要： In this study, a novel laccase gene, EuLAC1, was cloned from Eucommia ulmoides Oliver (E. ulmoides). An overexpression vector harboring the EuLAC1 was constructed and introduced into the tobacco (Nicotiana tabacum cv. Xanthi). The laccase activity, resistance to Botrytis cinerea (B. cinerea) and lignin level in wild-type and transgenic plants were thereafter investigated. Interestingly, the transgenic tobacco displayed a significantly higher laccase activity and resistance to gray mold as compared to the wild-type tobacco. Additionally, the lignin contents in the leaves and stems of the transgenic tobacco were significantly higher in comparison to the wild-type tobacco. Scanning electron microscopy was used to observe the cross sections of wild-type and transgenic tobacco stems and it was noted that the cell wall near the xylem catheter of the transgenic tobacco was substantially thicker and the outline clearer than that of the wild-type. Thus, the EuLAC1 gene can significantly increase laccase activity and lignin content in tobacco, leading to an increase in the physical defenses, thereby increasing tobacco resistance to gray mold.

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