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Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent "signal off" aptasensor for ochratoxin A detection in malt

文献类型： 外文期刊

作者： Zhang, Qing 1 ; Kang, Linzhi 3 ; Yue, Pengfei 1 ; Shi, Linchun 2 ; Wang, Meng 4 ; Zhou, Lidong 2 ; Zhao, Haiping 5 ; Kong, Weijun 2 ;

作者机构： 1.Jiangxi Univ Tradit Chinese Med, Pharm Coll, Key Lab Modern Preparat TCM, Minist Educ, Nanchang 330004, Jiangxi, Peoples R China

2.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China

3.Jiangxi Univ Tradit Chinese Med, Sch Nursing, Nanchang 330004, Jiangxi, Peoples R China

4.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China

5.Jiangxi Univ Tradit Chinese Med, Sch Tradit Chinese Med, Nanchang 330004, Jiangxi, Peoples R China

关键词： Ochratoxin A; Malt; Fluorescent aptasensor; Graphene oxide nanosheet; Double-stranded DNA

期刊名称：FOOD CHEMISTRY-X （ 影响因子：6.443； 五年影响因子：6.443 ）

ISSN： 2590-1575

年卷期： 2022 年 14 卷

页码：

收录情况： SCI

摘要： A "signal off" fluorescent aptasensor based on graphene oxide (GO) nanosheet and double-stranded DNA structure was fabricated for OTA detection. In the absence of OTA, the aptamer and its complementary DNA (cDNA) formed double-stranded conjugates that could coexist with GO, presenting fluorescence responses. Then, the presented OTA was captured by the aptamers, causing the release of cDNA-FAM probes. The free probes were adsorbed by GO, leading to an OTA concentration-dependent fluorescence quenching through fluorescence resonance energy transfer. Under optimum conditions, the fluorescent aptasensor exhibited outstanding sensitivity with a LOD of 11 pg/mL and a wide dynamic range of 0.04-30 ng/mL. The selectivity of the aptasensor was confirmed against other four mycotoxins, and the feasibility and reliability were verified by determining OTA in the spiked malt samples with satisfactory recovery of 95.50%-112.05%. This aptasensing platform could be adapted to measure other mycotoxins by replacing the aptamer sequences for food safety.

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