您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of a novel phage SPX1 and biological control for biofilm of Shewanella in shrimp and food contact surfaces

文献类型： 外文期刊

作者： Liu, Xi 1 ; Ming, Zixin 1 ; Ding, Yifeng 1 ; Guan, Peng 1 ; Shao, Yanchun 1 ; Wang, Lan 3 ; Wang, Xiaohong 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China

3.Hubei Acad Agr Sci, Minist Agr & Rural Affairs, Inst Agroprod Proc & Nucl Agr Technol, Key Lab Agr Prod Cold Chain Logist, Wuhan 430064, Peoples R China

关键词： Shewanella putrefaciens; Bacteriophage; Characteristics; Biofilms; Shrimp; Contact surface

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：5.3 ）

ISSN： 0168-1605

年卷期： 2025 年 426 卷

页码：

收录情况： SCI

摘要： Shewanella putrefaciens, , commonly found in seafood, forms tenacious biofilms on various surfaces, contributing to spoilage and cross-contamination. Bacteriophages, owing to their potent lytic capabilities, have emerged as novel and safe options for preventing and eliminating contaminants across various foods and food processing environments. In this study, a novel phage SPX1 was isolated, characterized by a high burst size (43.81 +/- 3.01 PFU/CFU) and a short latent period (10 min). SPX1 belongs to the Caudoviricetes class, exhibits resistance to chloroform, and sensitivity to ultraviolet. It shows stability over a wide range of temperatures (30-50 degrees C) and pH levels (3-11). The genome of phage SPX1 consists of 53,428 bp with 49.72 % G + C composition, and lacks tRNAs or virulence factors. Genome analysis revealed the presence of two endolysins, confirming its biofilmremoval capacity. Following the treatment of shrimp surface biofilm with the optimal MOI of 0.001 of phage SPX1 for 5 h, the bacterial count decreased by 1.84 +/- 0.1 log10 10 CFU/cm(2) (>98.5 %). Biofilms on the surfaces of the three common materials used in shrimp processing and transportation also showed varying degrees of reduction: glass (1.98 +/- 0.01 log10 10 CFU/cm(2)), stainless steel (1.93 +/- 0.05 log10 10 CFU/cm(2)), and polyethylene (1.38 +/- 0.1 log10 10 CFU/cm(2)). The study will contribute to phage as a novel and potent biocontrol agent for effectively managing S. putrefaciens and its biofilm, ensuring a reduction in spoilage bacteria contamination during the aquaculture, processing, and transportation of seafood products.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台