文献类型： 外文期刊

作者： Luo, Wen ¹ ; Li, Yuanli ¹ ; Luo, Ruiping ² ; Wei, Gehong ¹ ; Liu, Yongxin ³ ; Chen, Weimin ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Life Sci, State Key Lab Crop Stress Biol Arid Areas, Shaanxi Key Lab Agr & Environm Microbiol, Xianyang 712100, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Crop Res Inst, Yinchuan Comprehens Expt Stn Natl Soybean Ind Tech, Yinchuan 750105, Peoples R China

3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab,M, Shenzhen 518120, Peoples R China

关键词： Cuscuta; flavonoid metabolite; legume plant; parasitism status; rhizosphere; root-associated microbiome

期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN：

年卷期： 2023 年 13 卷 6 期

页码：

收录情况： SCI

摘要： Dodders (Cuscuta chinensis) are rootless and holoparasitic herbs that can infect a variety of host plants, including the vitally important economic and bioenergy crop soybean (Glycine max). Although dodder parasitism severely affects the physiology of host plants, little is known about its effects on fungal communities and root secondary metabolites in hosts. In this study, variations in root-associated fungal communities and root metabolites of soybean under different parasitism conditions were investigated using ITS rRNA gene sequencing and UPLC-MS/MS metabolome detection technologies. The results showed that dodder parasitism significantly altered the composition and diversity of the fungal communities in the rhizosphere and endosphere of soybean. The relative abundance of the potential pathogenic fungus Alternaria significantly increased in the root endosphere of dodder-parasitized soybean. Furthermore, correlation analysis indicated that the fungal community in the root endosphere was susceptible to soil factors under dodder parasitism. Meanwhile, the content of soil total nitrogen was significantly and positively correlated with the relative abundance of Alternaria in the rhizosphere and endosphere of soybean. Metabolomic analysis indicated that dodder parasitism altered the accumulation of flavonoids in soybean roots, with significant upregulation of the contents of kaempferol and its downstream derivatives under different parasitism conditions. Taken together, this study highlighted the important role of dodder parasitism in shaping the fungal communities and secondary metabolites associated with soybean roots, providing new insights into the mechanisms of multiple interactions among dodder, soybean, microbial communities and the soil environment.