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Natural variations in the P-type ATPase heavy metal transporter gene ZmHMA3 control cadmium accumulation in maize grains

文献类型： 外文期刊

作者： Tang, Bin 1 ; Luo, Meijie 2 ; Zhang, Yunxia 2 ; Guo, Huanle 1 ; Li, Jingna 2 ; Song, Wei 2 ; Zhang, Ruyang 2 ; Feng, Zhen 2 ;

作者机构： 1.Hunan Acad Agr Sci, Crop Res Inst, Changsha 410125, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing Key Lab Maize DNA Fingerprinting & Mol Br, Beijing 100097, Peoples R China

3.Qilu Normal Univ, Inst Mol Breeding Maize, Jinan 250200, Shandong, Peoples R China

4.China Agr Univ, Dept Plant Genet & Breeding, Beijing 100193, Peoples R China

5.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Germplasm Utilizat MOA, Beijing 100081, Peoples R China

6.Genetron Hlth Beijing Co Ltd, Beijing 102208, Peoples R China

关键词： Cadmium accumulation; grain; haplotype; maize; molecular markers; ZmHMA3

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2021 年 72 卷 18 期

页码：

收录情况： SCI

摘要： Cadmium (Cd) accumulation in maize grains is detrimental to human health. Developing maize varieties with low Cd content is important for safe consumption of maize grains. However, the key genes controlling maize grain Cd accumulation have not been cloned. Here, we identified one major locus for maize grain Cd accumulation (qCd1) using a genome-wide association study (GWAS) and bulked segregant RNA-seq analysis with a biparental segregating population of Jing724 (low-Cd line) and Mo17 (high-Cd line). The candidate gene ZmHMA3 was identified by fine mapping and encodes a tonoplast-localized heavy metal P-type ATPase transporter. An ethyl methane sulfonate mutant analysis and an allelism test confirmed that ZmHMA3 influences maize grain Cd accumulation. A transposon in intron 1 of ZmHMA3 is responsible for the abnormal amino acid sequence in Mo17. Based on the natural sequence variations in the ZmHMA3 gene of diverse maize lines, four PCR-based molecular markers were developed, and these were successfully used to distinguish five haplotypes with different grain Cd contents in the GWAS panel and to predict grain Cd contents of widely used maize inbred lines and hybrids. These molecular markers can be used to breed elite maize varieties with low grain Cd contents.

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