Overexpression of RmLCYB from Rhododendron molle increases carotenoid in Nicotiana tabacum
文献类型： 外文期刊
作者： Xiao, Zheng 1 ; Su, Jiale 1 ; Liu, Xiaoqing 1 ; Sun, Xiaobo 1 ; He, Lisi 1 ; Zhou, Huimin 1 ; Li, Chang 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Leisure Agr, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing, Jiangsu, Peoples R China
2.Zhejiang A&F Univ, Sch Landscape Architecture, Zhejiang Prov Key Lab Germplasm Innovat & Garden, Key Lab Natl Forestry & Grassland Adm Germplasm I, Hangzhou, Zhejiang, Peoples R China
关键词： Cloning; Rhododendron; LCYB; beta-Carotene; Flower coloration Introduction
期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.736； 五年影响因子：2.983 ）
ISSN： 0137-5881
年卷期： 2022 年 44 卷 7 期
页码：
收录情况： SCI
摘要： Rhododendron molle G. Don is a deciduous species of Rhododendron with unique yellow flowers. Carotenoids play a major role in flower coloration. Lycopene beta-cyclase (LCYB) is an essential enzyme which catalyzes the synthesis of beta-carotene from lycopene in the carotenoid biosynthesis pathway. However, the molecular function of the LCYB gene in R. molle remains unknown. In this study, the RmLCYB gene was first isolated from R. molle. The RmLCYB protein sequence showed high similarity with LCYB in other plant species. RmLCYB is expressed in petals during flower development and reaches the highest level at the initial flowering stage. The main content of carotenoid in R. molle flower is beta-carotene. To verify the function of RmLCYB, we overexpressed RmLCYB in tobacco. We found that the total content of carotenoid in the transgenic tobacco with overexpression of RmLCYB gene increased 1.73-fold compared with the wild-type tobacco. The results indicate that the RmLCYB gene can enhance the carotenoid content and alter flower color. This gene has the potential to promote carotenoid synthesis and improve our ability to create evergreen azaleas with desirable yellow flower.
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