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Effects of different salinity conditions on the transcriptome and antibiotic resistance of two Vibrio parahaemolyticus strains isolated from Penaeus vannamei cultured in seawater and freshwater ponds

文献类型： 外文期刊

作者： Ma, Rongrong 1 ; Wang, Yuan 1 ; Huang, Liang 1 ; Zhao, Shu 1 ; Li, Letian 1 ; Yin, Menghe 1 ; Fang, Wenhong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea Fishery Resources Exploita, Minist Agr, 300 Jungong Rd, Shanghai 200090, Peoples R China

2.Ningbo Univ, Coll Marine Sci, Ningbo, Peoples R China

关键词： drug susceptibility; salinity; transcriptome; Vibrio parahaemolyticus

期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）

ISSN： 0140-7775

年卷期： 2021 年 44 卷 12 期

页码：

收录情况： SCI

摘要： The transcriptome and antibiotic resistance of Vibrio parahaemolyticus isolated from Penaeus vannamei cultured in seawater (strain HN1)and freshwater (strain SH1) ponds were studied at different salinity (2 parts per thousand and 20 parts per thousand). At different salinity, 623 differentially expressed genes (DEGs) significantly upregulated and 1,559 DEGs significantly downregulated in SH1. In HN1, 466 DEGs significantly upregulated and 1,930 DEGs significantly downregulated, indicating high salinity can lead to the downregulation of most genes. In KEGG analysis, the expression of DEGs annotated to starch and sucrose metabolism pathway was higher at 2 parts per thousand salinity than at 20 parts per thousand salinity in HN1 and SH1, implying salinity affected bacterial growth mainly through this pathway. In the enrichment analysis of upregulated DEGs, two pathways (Valine, leucine, and isoleucine degradation, and Butanoate metabolism) were significantly enriched at different salinity. Antibiotic-susceptibility test discovered that SH1 isolated from P. vannamei cultured in freshwater was resistant to multiple drugs, including kanamycin, gentamicin, medemycin, and azithromycin, at a salinity of 2 parts per thousand, whereas at 20 parts per thousand salinity, SH1 was not resistant to the drugs. The HN1 strain isolated from P. vannamei cultured in mariculture was resistant to polymyxin B and clindamycin at 20 parts per thousand salinity. Whereas, HN1 was intermediately susceptible to these two antibiotics at 2 parts per thousand salinity. These results indicate that the drug resistance of bacteria was affected by salinity. Furthermore, beta-lactam resistance was significantly enriched in SH1 at different salinity, and the inhibition zone of penicillin G was consistent with the results of a beta-lactam resistance pathway.

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