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Expression pattern of diacylglycerol acyltransferase-1, an enzyme involved in triacylglycerol biosynthesis, in Arabidopsis thaliana

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文献类型：外文期刊

作者： Lu, CFL ¹ ; de Noyer, SB ² ; Hobbs, DH ² ; Kang, JL ² ; Wen, YC ² ; Krachtus, D ² ; Hills, MJ ² ;

作者机构： 1.John Innes Ctr, Norwich NR4 7UH, Norfolk, England

2.John Innes Ctr, Norwich NR4 7UH, Norfolk, England; Henan Acad Agr Sci, Zhenzhou, Henan Province, Peoples R China

关键词： Arabidopsis;Diacylglycerol acyltransferase;Expression pattern;Beta-glucuronidase (gus);Triacylglycerol synthesis;Brassica-napus;Abscisic-acid;Functional dissection;Gene-expression;Higher-plants;Ry repeat;Promoter;Protein;Seed;Elements

期刊名称：PLANT MOLECULAR BIOLOGY （影响因子：4.076；五年影响因子：4.89 ）

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收录情况： SCI

摘要： Triacylglycerol (TAG) is the major carbon storage reserve in oilseeds such as Arabidopsis. Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyses the final step of the TAG synthesis pathway. Although TAG is mainly accumulated during seed development, and DGAT has presumably the highest activity in developing seeds, we show here that TAG synthesis is also actively taking place during germination and seedling development in Arabidopsis. The expression pattern of the DGAT1 gene was studied in transgenic plants containing the reporter gene beta-glucuronidase (GUS) fused with DNA sequences flanking the DGAT1 coding region. GUS activity was not only detected in developing seeds and pollen, which normally accumulate storage TAG, but also in germinating seeds and seedlings. Western blots showed that DGAT1 protein is present in several tissues, though is most abundant in developing seeds. In seedlings, DGAT1 is expressed in shoot and root apical regions, correlating with rapid cell division and growth. The expression of GUS in seedlings was consistent with the results of RNA gel blot analyses, precursor feeding and DGAT assay. In addition, DGAT1 gene expression is up-regulated by glucose and associated with glucose-induced changes in seedling development. [References: 48]

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Expression pattern of diacylglycerol acyltransferase-1, an enzyme involved in triacylglycerol biosynthesis, in Arabidopsis thaliana

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Expression pattern of diacylglycerol acyltransferase-1, an enzyme involved in triacylglycerol biosynthesis, in Arabidopsis thaliana

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