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SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS OF RT-PCR PRODUCTS OF UK ISOLATES OF BARLEY YELLOW MOSAIC VIRUS

文献类型: 外文期刊

作者: Shi, NN 1 ; Chen, JP 2 ; Wilson, TMA 2 ; Macfarlane, SA 2 ; Antoniw, JF 2 ; Adams, MJ 3 ;

作者机构: 1.AFRC,INST ARABLE CROPS RES,CROP & DIS MANAGEMENT DEPT,HARPENDEN AL5 2JQ,HERTS,ENGLAND

2.AFRC,INST ARABLE CROPS RES,CROP & DIS MANAGEMENT DEPT,HARPENDEN AL5 2JQ,HERTS,ENGLAND; SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND; ZHEJIANG ACAD AGR SCI,VIROL LAB,HANGZHOU 310021,PEOPLES R CHINA

3.AFRC,INST ARABLE CROPS RES,CROP & DIS MANAGEMENT DEPT,HARPENDEN AL5 2JQ,HERTS,ENGLAND; SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND; ZH

关键词: Barley yellow mosaic virus;Single strand conformation polymorphism.;Nucleotide sequence;Point mutations;Gene;Rna 2

期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Single-strand conformation polymorphism (SSCP) analysis of the bipartite genomes of several UK isolates of barley yellow mosaic virus (BaYMV) was done using fragments of cDNA amplified by RT-PCR. Isolated differed in their SSCP patterns in several regions, but in no case was the pattern able to distinguish between common and resistance-breaking strains. In regions where the nucleotide sequences of UK isolates had been determined, there was no simple relationship between numbers of nucleotide differences and SSCP patterns: differences of only 2 or 3 nucleotides (nt) gave different SSCP patterns, whereas differences of as many as 29 nt did not. Although SSCP analysis has some potential as a rapid and sensitive tool for distinguishing virus isolates, differences detected do not necessarily relate to biological properties and the results are highly dependent on gel conditions.

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