文献类型： 外文期刊
作者： Yang, Wen-Qing 1 ; Xiong, Qing-Ping 2 ; Ge, Jian-Yang 1 ; Li, Hao 1 ; Zhu, Wen-Yu 1 ; Nie, Yan 4 ; Lin, Xiuying 5 ; Lv, Daizhu 6 ; Li, Jing 1 ; Lin, Huan 5 ; Liu, Ru-Juan 1 ;
作者机构： 1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
2.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
3.Univ Chinese Acad Sci, Shanghai 200031, Peoples R China
4.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China
5.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou, Hainan, Peoples R China
6.Chinese Acad Trop Agr Sci, Anal & Testing Ctr, Haikou 571101, Hainan, Peoples R China
期刊名称：NUCLEIC ACIDS RESEARCH （ 影响因子：19.16； 五年影响因子：17.21 ）
ISSN： 0305-1048
年卷期： 2021 年 49 卷 20 期
页码：
收录情况： SCI
摘要： Post-transcriptional modifications affect tRNA biology and are closely associated with human diseases. However, progress on the functional analysis of tRNA modifications in metazoans has been slow because of the difficulty in identifying modifying enzymes. For example, the biogenesis and function of the prevalent N2-methylguanosine (m(2)G) at the sixth position of tRNAs in eukaryotes has long remained enigmatic. Herein, using a reverse genetics approach coupled with RNA-mass spectrometry, we identified that THUMP domain-containing protein 3 (THUMPD3) is responsible for tRNA: m(2)G6 formation in human cells. However, THUMPD3 alone could not modify tRNAs. Instead, multifunctional methyltransferase subunit TRM112-like protein (TRMT112) interacts with THUMPD3 to activate its methyltransferase activity. In the in vitro enzymatic assay system, THUMPD3-TRMT112 could methylate all the 26 tested G6-containing human cytoplasmic tRNAs by recognizing the characteristic 3'-CCA of mature tRNAs. We also showed that m(2)G7 of tRNA(Trp) was introduced by THUMPD3-TRMT112. Furthermore, THUMPD3 is widely expressed in mouse tissues, with an extremely high level in the testis. THUMPD3-knockout cells exhibited impaired global protein synthesis and reduced growth. Our data highlight the significance of the tRNA: m(2)G6/7 modification and pave a way for further studies of the role of m(2)G in sperm tRNA derived fragments.
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