Exploring urinary modified nucleosides as biomarkers for diabetic retinopathy: Development and validation of a ultra performance liquid chromatography-tandem mass spectrometry method
文献类型： 外文期刊
作者： Yao, Chen 1 ; Lv, Daizhu 3 ; Zhou, Xueqing 4 ; Fu, Pengcheng 1 ; Sun, Wen 5 ; Chen, Jinlian 6 ; Lin, Huan 1 ;
作者机构： 1.Hainan Univ, State Key Lab Marine Resource Utilizat South China, Haikou 570228, Peoples R China
2.Hainan Univ, Sch Life Sci, Haikou 570228, Peoples R China
3.Chinese Acad Trop Agr Sci, Anal & Testing Ctr, Haikou 571101, Peoples R China
4.Hainan Univ, Anal & Testing Ctr, Haikou 570228, Hainan, Peoples R China
5.Hainan Tradit Chinese Med Hosp, Hosp Chinese Med, Hainan Med Coll, Haikou 570203, Peoples R China
6.Sun Yat Sen Univ, Hainan Eye Hosp, Zhongshan Ophthalm Ctr, Haikou 570311, Peoples R China
7.Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, Key Lab Ophthalmol, Haikou 570311, Peoples R China
关键词： UPLC-MS/MS; Modified nucleosides; Diabetic retinopathy; Biomarkers
期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.0； 五年影响因子：2.9 ）
ISSN： 1570-0232
年卷期： 2024 年 1232 卷
页码：
收录情况： SCI
摘要： The dynamic modification of RNA plays a crucial role in biological regulation and is strongly linked to human disease development and progression. Notably, modified nucleosides in urine have shown promising potential as early diagnostic biomarkers for various conditions. In this study, we developed and validated a rapid, sensitive, and accurate UPLC-MS/MS method for quantifying eight types of modified nucleosides (N-1-methyladenosine (m(1)A), N-6-methyladenosine (m(6)A), 5-methyluridine (m(5)U), 5-taurinomethyl-2-thiouridine (tau m(5)s(2)U), 5-methylcytidine (m(5)C), 2'-O-methylcytidine (Cm), N-1-methylguanosine (m(1)G), and N-7-methylguanosine (m(7)G) in human urine. Using the method, we measured the urinary concentrations of m(1)A, m(6)A, m(5)U, tau m(5)s(2)U, m(5)C, Cm, m(1)G, and m(7)G in a total of 21 control individuals and 23 patients diagnosed with diabetic retinopathy (DR). Cm levels showed promise as a diagnostic marker for diabetic retinopathy (DR), with a significant value (P < 0.01) and an AUC of 0.735. Other modified nucleosides also exhibited significant differences within specific subpopulations. As non-proliferative diabetic retinopathy (NPDR) signifies the latent early stage of diabetic retinopathy, we developed a multivariate linear model that integrates patients' sex, age, height, and urinary concentration of modified nucleosides which aims to predict and differentiate between healthy individuals, NPDR patients, and proliferative diabetic retinopathy (PDR) patients. Encouragingly, the model achieved satisfactory accuracy rates: healthy (81%), NPDR (75%), and PDR (80%). Our findings provide valuable insights into the development of an early, cost-effective, and noninvasive diagnostic approach for diabetic retinopathy.
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