文献类型： 外文期刊
作者： Jiang, Na 1 ; Yuan, Ding 1 ; Zhang, Min 2 ; Luo, Lin 1 ; Wang, Na 2 ; Xing, Wei 1 ; Li, Tieliang 1 ; Huang, Xiaoli 3 ; Ma, Zhihong 1 ;
作者机构： 1.Beijing Fisheries Res Inst, Beijing Key Lab Fishery Biotechnol, Beijing 100068, Peoples R China
2.Chinese Acad Inspect & Quarantine, Inst Anim Quarantine, Beijing, Peoples R China
3.Sichuan Agr Univ, Dept Aquaculture, Huimin Rd 211, Wenjiang 611130, Sichuan, Peoples R China
关键词： Cyprinid herpesvirus 2; Goldfish; Histology; Electron microscopy; Detection
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2020 年 523 卷
页码：
收录情况： SCI
摘要： A lethal disease of goldfish (Carassius auratus) afflicted ornamental fish farms in Beijing in 2018, causing severe financial losses. Morbidity and mortality are restricted to goldfish and occurred in spring and autumn, when water temperature was between 15 and 28 degrees C. Moribund goldfish from ornamental fish farms were tested for Cyprinid herpesvirus 2 (CyHV-2) by polymerase chain reaction (PCR) and real-time PCR using the viral polymerase gene followed by sequence analysis. The polymerase gene-specific product shared 99.10% nucleotide sequence identity to the published sequence of CyHV-2. Histology and electron microscopy showed that the moribund fish had severe necrosis in the gill and kidney and herpesvirus particles were demonstrated by Transmission Electron Microscopy(TEM). We infer, from these findings, that CyHV-2 might be transferred systemically via blood circulation system and that goldfish herpesviral hematopoietic necrosis is likely to be an important or serious infection disease for goldfish farms in this region of China.
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