文献类型： 外文期刊

作者： Tang, Shu-Kun ¹ ; Zhi, Xiao-Yang ¹ ; Zhang, Yao ² ; Makarova, Kira S. ³ ; Liu, Bing-Bing ¹ ; Zheng, Guo-Song ⁵ ; Zhang, Zhen-Peng ² ; Zheng, Hua-Jun ⁶ ; Wolf, Yuri I. ³ ; Zhao, Yu-Rong ¹ ; Jiang, Song-Hao ² ; Chen, Xi-Ming ⁹ ; Li, En-Yuan ¹ ; Zhang, Tao ² ; Chen, Pei-Ru ² ; Feng, Yu-Zhou ¹ ; Xiang, Ming-Xian ¹ ; Lin, Zhi-Qian ¹ ; Shi, Jia-Hui ² ; Chang, Cheng ² ; Zhang, Xue ⁴ ; Li, Rui ¹ ; Lou, Kai ¹⁰ ; Wang, Yun ¹⁰ ; Chang, Lei ² ; Yin, Min ¹ ; Yang, Ling-Ling ¹ ; Gao, Hui-Ying ² ; Zhang, Zhong-Kai ¹¹ ; Tao, Tian-Shen ¹² ; Guan, Tong-Wei ¹³ ; He, Fu-Chu ² ; Lu, Yin-Hua ⁵ ; Cui, Heng-Lin ¹⁴ ; Koonin, Eugene V. ³ ; Zhao, Guo-Ping ¹⁵ ; Xu, Ping ² ;

作者机构： 1.Yunnan Univ, Yunnan Inst Microbiol, Sch Life Sci, Key Lab Microbial Resources,Minist Educ, Kunming 650091, Peoples R China

2.Chinese Acad Med Sci, Inst Life, Beijing Proteome Res Ctr, Natl Ctr Prot Sci Beijing,Res Unit Prote & Res &, Beijing 102206, Peoples R China

3.NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, 8600 Rockville Pike, Bethesda, MD 20894 USA

4.Nanyang Inst Technol, Coll Biol & Chem Engn, Henan Key Lab Ind Microbial Resources & Fermentat, Nanyang 473004, Peoples R China

5.Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China

6.Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai, Peoples R China

7.Shanghai Inst Biomed & Pharmaceut Technol, Shanghai 201203, Peoples R China

8.Hebei Univ, Coll Life Sci, Hebei Prov Key Lab Res & Applicat Microbial Diver, Baoding 071002, Hebei, Peoples R China

9.Chinese Acad Sci, Northwest Inst Eco Environm & Resources, Key Lab Desert & Desertificat, Lanzhou 730000, Peoples R China

10.Xinjiang Acad Agr Sci, Xinjiang Inst Microbiol, Urumqi 830091, Peoples R China

11.Yunnan Acad Agr Sci, Biotechnol & Genet Germplasm Resources Res Inst, Kunming 650205, Peoples R China

12.Wuhan Univ, Sch Pharmaceut Sci, Dept Microbiol, Key Lab Combinatorial Biosynth & Drug Discovery,M, Wuhan 430072, Peoples R China

13.Xihua Univ, Coll Food & Biol Engn, Chengdu 610039, Peoples R China

14.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

15.Fudan Univ, Sch Basic Med Sci, Key Lab Med Mol Virol, MOE,NHC,CAMS, Shanghai 200032, Peoples R China

16.Fudan Univ, Sch Life Sci, Dept Microbiol & Microbial Engn, Shanghai 200032, Peoples R China

17.Guizhou Univ, Sch Med, Guiyang 550025, Peoples R China

18.Guangzhou Univ Chinese Med, Affiliated Hosp 2, State Key Lab Dampness Syndrome Chinese Med, Guangzhou 510120, Peoples R China

期刊名称：NATURE COMMUNICATIONS （ 影响因子：16.6； 五年影响因子：17.0 ）

ISSN：

年卷期： 2023 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Several groups of bacteria have complex life cycles involving cellular differentiation and multicellular structures. For example, actinobacteria of the genus Streptomyces form multicellular vegetative hyphae, aerial hyphae, and spores. However, similar life cycles have not yet been described for archaea. Here, we show that several haloarchaea of the family Halobacteriaceae display a life cycle resembling that of Streptomyces bacteria. Strain YIM 93972 (isolated from a salt marsh) undergoes cellular differentiation into mycelia and spores. Other closely related strains are also able to form mycelia, and comparative genomic analyses point to gene signatures (apparent gain or loss of certain genes) that are shared by members of this clade within the Halobacteriaceae. Genomic, transcriptomic and proteomic analyses of non-differentiating mutants suggest that a Cdc48-family ATPase might be involved in cellular differentiation in strain YIM 93972. Additionally, a gene encoding a putative oligopeptide transporter from YIM 93972 can restore the ability to form hyphae in a Streptomyces coelicolor mutant that carries a deletion in a homologous gene cluster (bldKA-bldKE), suggesting functional equivalence. We propose strain YIM 93972 as representative of a new species in a new genus within the family Halobacteriaceae, for which the name Actinoarchaeum halophilum gen. nov., sp. nov. is herewith proposed. Our demonstration of a complex life cycle in a group of haloarchaea adds a new dimension to our understanding of the biological diversity and environmental adaptation of archaea. Bacteria of the genus Streptomyces have complex life cycles involving cellular differentiation and multicellular structures that have never been observed in archaea. Here, the authors show that several halophilic archaea display a life cycle resembling that of Streptomyces bacteria, undergoing cellular differentiation into mycelia and spores.