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Cloning of Salt Tolerance-Related cDNAs from the Mangrove Plant Sesuvium portulacastrum L.

文献类型： 外文期刊

作者： Zeng, Hui-Cai 1 ; Deng, Liu-Hong 2 ; Zhang, Chun-Fa 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China

2.Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Inst Trop Biosci & Biotechnol, Kaikou 571101, Peoples R China; CATAS, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China

关键词： mangrove;salt tolerance-related cDNAs;Sesuvium portulacastrum;suppressive subtractive hybridization

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

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收录情况： SCI

摘要： In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant (Sesuvium portulacastrum L.), a subtracted cDNA library was constructed via suppressive subtractive hybridization (SSH), in which the poly(A)+RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly(A)+RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant (LEA) proteinof A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants.

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