文献类型： 外文期刊

作者： Wei, Taiyun ¹ ; Kikuchi, Akira ² ; Moriyasu, Yusuke ² ; Suzuki, Nobuhiro ³ ; Shimizu, Takumi; Hagiwara, Kyoji; C ¹ ;

作者机构： 1.Natl Agr Res Ctr, Virol Lab, Tsukuba, Ibaraki 3058666, Japan

2.Natl Agr Res Ctr, Virol Lab, Tsukuba, Ibaraki 3058666, Japan; Ibaraki Univ, Fac Agr, Ami, Ibaraki 3000332, Japan; Okayama Univ, Bioresources Res Inst, Kurashiki, Okayama 7100046, Japan; Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan; Fujian Acad Agr Sci, Ctr Biotechnol, Fuzhou 350003, Fujian, Peoples R China

3.Natl Agr Res Ctr, Virol Lab, Tsukuba, Ibaraki 3058666, Japan; Ibaraki Univ, Fac Agr, Ami, Ibaraki 3000332, Japan; Okayama Univ, Bioresources Res Inst, Kurashiki, Okayama 7100046, Japan; Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan; Fujian Acad Agr Sci, Ctr Biotechnol, Fuzhou 350003, Fujian, Peoples

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）

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收录情况： SCI

摘要： Various cytopathological structures, known as inclusion bodies, are formed upon infection of cultured leafhopper cells by Rice dwarf virus, a member of the family Reoviridae. These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles. Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus. These tubules, when associated with actin-based filopodia, were able to protrude from the surface of cells and to penetrate neighboring cells. A binding assay in vitro revealed the specific binding of Pns10 to actin. Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus, even in the presence of neutralizing antibodies. However, treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells, with a significant simultaneous decrease in the extent of infection of neighboring cells. These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus, wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells.