文献类型： 外文期刊

作者： Wei, Taiyun ¹ ; Chen, Hongyan ² ; Ichiki-Uehara, Tamaki ² ; Hibino, Hiroyuki ² ; Omura, Toshihiro ² ;

作者机构： 1.Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan

2.Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan; Natl Agr Res Ctr, Virol Lab, Tsukuba, Ibaraki 3058666, Japan; Fujian Acad Agr Sci, Ctr Biotechnol, Fuzhou 350003, Peoples R China

关键词： OUTER CAPSID PROTEIN;MAJOR STRUCTURAL PROTEINS;P2 PROTEIN;PHYTOREOVIRUS;ENDOSOMES;MECHANISM;INFECTION;CAVEOLAE;REVEALS

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）

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收录情况： SCI

摘要： Electron microscopy revealed that the entry of Rice dwarf virus (RDV) into insect vector cells involved endocytosis via coated pits. The treatment of cells with drugs that block receptor-mediated or clathrin-mediated endocytosis significantly reduced RDV infectivity. However, the drug that blocks caveola-mediated endocytosis had a negligible effect on such infection. Infection was also inhibited when cells had been pretreated with bafilomycin A1, which interferes with acidification of endosomes. Moreover, immunofluorescence staining indicated that the virus is internalized into early endosomes. Together, our data indicate that RDV enters insect vector cells through receptor-mediated, clathrin-dependent endocytosis and is sequestered in early endosomes.